Endoglin/CD105 Polyclonal antibody

Endoglin/CD105 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10862-1-AP

Synonyms

CD105, END, Endoglin, ENG, HHT1, ORW, ORW1



Tested Applications

Positive WB detected inhuman placenta tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman breast cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

10862-1-AP targets Endoglin/CD105 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity Bovine, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Endoglin/CD105 fusion protein Ag1287
Full Name endoglin
Calculated molecular weight 71 kDa
Observed molecular weight 75-90 kDa, 170-180 kDa
GenBank accession numberBC014271
Gene symbol ENG
Gene ID (NCBI) 2022
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

Protocols

Product Specific Protocols
WB protocol for Endoglin/CD105 antibody 10862-1-APDownload protocol
IHC protocol for Endoglin/CD105 antibody 10862-1-APDownload protocol
IP protocol for Endoglin/CD105 antibody 10862-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Eur Rev Med Pharmacol Sci

MMP-14 aggravates onset of severe preeclampsia by mediating soluble endoglin release.

Authors - X-H Zhang
BovineIF

J Exp Zool B Mol Dev Evol

Inhibiting transforming growth factor-β signaling regulates in vitro maintenance and differentiation of bovine bone marrow mesenchymal stem cells.

Authors - Xin-Xin Zhao

Cell Reprogram

Inhibition of DNA Methyltransferase by RG108 Promotes Pluripotency-Related Character of Porcine Bone Marrow Mesenchymal Stem Cells.

Authors - Qi Li
humanIHC

Placenta

Placental expression of anti-angiogenic proteins in mirror syndrome: a case report.

Authors - Graham N N
humanIHC

Placenta

Imbalance of angiogenic factors and avascular edematous cystic villi in a trisomy 13 pregnancy: a case report.

Authors - Kakigano A A
humanIHC

Mol Hum Reprod

Oxidative stress-induced S100B protein from placenta and amnion affects soluble Endoglin release from endothelial cells.

Authors - Tskitishvili E E