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  • KD/KO Validated

Endoglin/CD105 Polyclonal antibody

Endoglin/CD105 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10862-1-AP

Synonyms

CD105, END, Endoglin, ENG, HHT1, ORW, ORW1



Tested Applications

Positive WB detected inhuman placenta tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman breast cancer tissue, rat kidney tissue, human placenta tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

10862-1-AP targets Endoglin/CD105 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, Bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Endoglin/CD105 fusion protein Ag1287
Full Name endoglin
Calculated molecular weight 71 kDa
Observed molecular weight 75-90 kDa, 170-180 kDa
GenBank accession numberBC014271
Gene symbol ENG
Gene ID (NCBI) 2022
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

Protocols

Product Specific Protocols
WB protocol for Endoglin/CD105 antibody 10862-1-APDownload protocol
IHC protocol for Endoglin/CD105 antibody 10862-1-APDownload protocol
IP protocol for Endoglin/CD105 antibody 10862-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancers (Basel)

WISP2/CCN5 Suppresses Vasculogenic Mimicry through Inhibition of YAP/TAZ Signaling in Breast Cancer Cells.

Authors - Nathalie Ferrand
humanWB

Int J Biol Sci

Roquin2 suppresses breast cancer progression by inhibiting tumor angiogenesis via selectively destabilizing proangiogenic factors mRNA.

Authors - Meicen Zhou
humanWB

Biomedicines

Endoglin Modulates TGFβR2 Induced VEGF and Proinflammatory Cytokine Axis Mediated Angiogenesis in Prolonged DEHP-Exposed Breast Cancer Cells.

Authors - Mahendra Jadhao
  • KD Validated
humanIHC

Mol Hum Reprod

Oxidative stress-induced S100B protein from placenta and amnion affects soluble Endoglin release from endothelial cells.

Authors - Tskitishvili E E
human,mouseWB,IHC

Exp Cell Res

Human umbilical cord mesenchymal stem cells regulate CD54 and CD105 in vascular endothelial cells and suppress inflammation in Kawasaki disease.

Authors - Tao Chen
ratIF

World J Stem Cells

Urolithin a alleviates oxidative stress-induced senescence in nucleus pulposus-derived mesenchymal stem cells through SIRT1/PGC-1α pathway.

Authors - Peng-Zhi Shi