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  • KD/KO Validated

Endoglin/CD105 Polyclonal antibody

Endoglin/CD105 Polyclonal Antibody for WB, IP, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 10862-1-AP


CD105, END, Endoglin, ENG, HHT1, ORW, ORW1

Tested Applications

Positive WB detected inHUVEC cells, human placenta tissue, mouse lung tissue, rat lung tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman breast cancer tissue, rat kidney tissue, human placenta tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inTHP-1 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10862-1-AP targets Endoglin/CD105 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, Bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Endoglin/CD105 fusion protein Ag1287
Full Name endoglin
Calculated Molecular Weight 71 kDa
Observed Molecular Weight 75-90 kDa, 170-180 kDa
GenBank Accession NumberBC014271
Gene Symbol ENG
Gene ID (NCBI) 2022
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)


Product Specific Protocols
WB protocol for Endoglin/CD105 antibody 10862-1-APDownload protocol
IHC protocol for Endoglin/CD105 antibody 10862-1-APDownload protocol
IP protocol for Endoglin/CD105 antibody 10862-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cancers (Basel)

WISP2/CCN5 Suppresses Vasculogenic Mimicry through Inhibition of YAP/TAZ Signaling in Breast Cancer Cells.

Authors - Nathalie Ferrand

Int J Biol Sci

Roquin2 suppresses breast cancer progression by inhibiting tumor angiogenesis via selectively destabilizing proangiogenic factors mRNA.

Authors - Meicen Zhou


Endoglin Modulates TGFβR2 Induced VEGF and Proinflammatory Cytokine Axis Mediated Angiogenesis in Prolonged DEHP-Exposed Breast Cancer Cells.

Authors - Mahendra Jadhao
  • KD Validated

Mol Hum Reprod

Oxidative stress-induced S100B protein from placenta and amnion affects soluble Endoglin release from endothelial cells.

Authors - Tskitishvili E E

World J Stem Cells

Quercetin ameliorates oxidative stress-induced senescence in rat nucleus pulposus-derived mesenchymal stem cells via the miR-34a-5p/SIRT1 axis

Authors - Wen-Jie Zhao

Exp Cell Res

Human umbilical cord mesenchymal stem cells regulate CD54 and CD105 in vascular endothelial cells and suppress inflammation in Kawasaki disease.

Authors - Tao Chen