|Positive WB detected in||human placenta tissue|
|Positive IP detected in||mouse lung tissue|
|Positive IHC detected in||human breast cancer tissue, human placenta tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Sample-dependent, check data in validation data gallery|
10862-1-AP targets Endoglin/CD105 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||Bovine, human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Endoglin/CD105 fusion protein Ag1287|
|Calculated molecular weight||71 kDa|
|Observed molecular weight||75-90 kDa, 170-180 kDa|
|GenBank accession number||BC014271|
|Gene ID (NCBI)||2022|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)
Eur Rev Med Pharmacol Sci
MMP-14 aggravates onset of severe preeclampsia by mediating soluble endoglin release.
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Inhibiting transforming growth factor-β signaling regulates in vitro maintenance and differentiation of bovine bone marrow mesenchymal stem cells.
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Umbilical artery tissue contains p75 neurotrophin receptor-positive pericyte-like cells that possess neurosphere formation capacity and neurogenic differentiation potential.
Placental expression of anti-angiogenic proteins in mirror syndrome: a case report.
Imbalance of angiogenic factors and avascular edematous cystic villi in a trisomy 13 pregnancy: a case report.