ENO1 Polyclonal antibody

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  WB analysis of HeLa using 55237-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55237-1-AP (ENO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 55237-1-AP

  WB result of ENO1 antibody (55237-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ENO1 transfected HEK-293 cells.

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  WB analysis of HeLa using 55237-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55237-1-AP (ENO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 55237-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55237-1-AP (ENO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 55237-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55237-1-AP (ENO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 55237-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55237-1-AP (ENO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 55237-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55237-1-AP (ENO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 55237-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55237-1-AP (ENO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 55237-1-AP

  IP result of anti-ENO1 (IP:55237-1-AP, 4ug; Detection:55237-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human brain using 55237-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 55237-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 55237-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 55237-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skeletal muscle using 55237-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 55237-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human pancreas using 55237-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas using 55237-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human pancreas using 55237-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas using 55237-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 55237-1-AP

  Immunofluorescent analysis of HepG2 cells using 55237-1-AP (ENO1 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

• Featured Product
• KD/KO Validated

ENO1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 55237-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Enolase 1, ENO1L1, EC:4.2.1.11, C-myc promoter-binding protein, Alpha-enolase

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, HepG2 cells, mouse liver tissue, HEK-293 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human brain tissue, human pancreas tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IP: See 1 publications below

Product Information

55237-1-AP targets ENO1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: enolase 1, (alpha)
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: NM_001428
• Gene Symbol: ENO1
• Gene ID (NCBI): 2023
• ENSEMBL Gene ID: ENSG00000074800
• RRID: AB_10837362
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P06733
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ENO1, also named as NNE, ENO1L1, MBPB1, MPB1 and MBP1, belongs to the enolase family. ENO1 is a metabolic enzyme involved in the synthesis of pyruvate. It also acts as a plasminogen receptor and mediates the activation of plasmin and extracellular matrix degradation. In tumor cells, ENO1 is up-regulated and supports the Warburg effect; it is expressed at the cell surface, where it promotes cancer invasion, and is subjected to a specific array of post-translational modifications, namely acetylation, methylation and phosphorylation. ENO1 overexpression and post-translational modifications could be of diagnostic and prognostic value in many cancer types. (PMID: 27814656). This antibody is specific to ENO1 and has no cross reaction with ENO2 and ENO3.

Publications

Species	Application	Title
human	WB,IP	EMBO Rep; Histone acetyltransferase 1 is a succinyltransferase for histones and non-histones and promotes tumorigenesis.; Authors - Guang Yang