ENO1 Polyclonal antibody
ENO1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 55237-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HepG2 cells, mouse brain tissue, mouse liver tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human brain tissue, human pancreas tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 1 publications below |
IP | See 1 publications below |
Product Information
55237-1-AP targets ENO1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | enolase 1, (alpha) |
Calculated molecular weight | 47 kDa |
Observed molecular weight | 47 kDa |
GenBank accession number | NM_001428 |
Gene symbol | ENO1 |
Gene ID (NCBI) | 2023 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
ENO1, also named as NNE, ENO1L1, MBPB1, MPB1 and MBP1, belongs to the enolase family. It is a multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. ENO1 may also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. It stimulates immunoglobulin production. (PMID:2005901). This antibody is specific to ENO1. It has no cross reaction to ENO2 and ENO3.
Protocols
Product Specific Protocols | |
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WB protocol for ENO1 antibody 55237-1-AP | Download protocol |
IHC protocol for ENO1 antibody 55237-1-AP | Download protocol |
IF protocol for ENO1 antibody 55237-1-AP | Download protocol |
IP protocol for ENO1 antibody 55237-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
EMBO Rep Histone acetyltransferase 1 is a succinyltransferase for histones and non-histones and promotes tumorigenesis. |