ENO3 Polyclonal antibody, PBS Only

• 

  WB analysis of human skeletal muscle using 16421-1-AP (same clone as 16421-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16421-1-AP (ENO3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16421-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 16421-1-AP (same clone as 16421-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 16421-1-AP (ENO3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16421-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 16421-1-AP (same clone as 16421-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ENO3 antibody (16421-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 16421-1-PBS in a different storage buffer formulation.

ENO3 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 16421-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Muscle-specific enolase, MSE, GSD13, EC:4.2.1.11, Beta-enolase

---

Product Information

16421-1-PBS targets ENO3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9613

  Product name: Recombinant human ENO3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 94-434 aa of BC017249

  Sequence: MIELDGTENKSKFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGNPDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMRIGAEVYHHLKGVIKAKYGKDATNVGDEGGFAPNILENNEALELLKTAIQAAGYPDKVVIGMDVAASEFYRNGKYDLDFKSPDDPARHITGEKLGELYKSFIKNYPVVSIEDPFDQDDWATWTSFLSGVNIQIVGDDLTVTNPKRIAQAVEKKACNCLLLKVNQIGSVTESIQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEALGDKAIFAGRKFRNPKAK

• Full Name: enolase 3 (beta, muscle)
• Calculated Molecular Weight: 434 aa, 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC017249
• Gene Symbol: ENO3
• Gene ID (NCBI): 2027
• RRID: AB_2099464
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P13929
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ENO3, also named as MSE, belongs to the enolase family. It appears to have a function in striated muscle development and regeneration. Defects in ENO3 are the cause of glycogen storage disease type 13 (GSD13).