EPB42 Polyclonal antibody

EPB42 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27443-1-AP

Synonyms

E42P, EPB42, Erythrocyte protein 4.2, P4.2, PA, SPH5

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Validation Data Gallery

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  WB analysis using 27443-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27443-1-AP (EPB42 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27443-1-AP targets EPB42 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EPB42 fusion protein Ag23556
• Full Name: erythrocyte membrane protein band 4.2
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC096093
• Gene Symbol: EPB42
• Gene ID (NCBI): 2038
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EPB42, also known as Protein 4.2, is amongst the most abundant protein components of the erythrocyte membrane, representing approximately 5% membrane content or ~2.5 × 105 copies/cell. EPB42 binds to the cytoplasmic domain of band 3, which serves as a site for the anchorage of several membrane skeletal proteins(PMID: 19269200, 12049649). EPB42 is a 72 kDa peripheral membrane protein.