EPB42 Polyclonal antibody

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  WB analysis using 27443-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27443-1-AP (EPB42 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27443-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27443-1-AP (EPB42 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 27443-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27443-1-AP (EPB42 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 27443-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27443-1-AP (EPB42 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

EPB42 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27443-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

SPH5, Protein 4.2, PA, P4.2, Erythrocyte protein 4.2

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Tested Applications

• Positive WB detected in: human placenta tissue, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27443-1-AP targets EPB42 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag23556

  Product name: Recombinant human EPB42 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 446-691 aa of BC096093

  Sequence: EVLERVEKEKMEREKDNGIRPPSLETASPLYLLLKAPSSLPLRGDAQISVTLVNHSEQEKAVQLAIGVQAVHYNGVLAAKLWRKKLHLTLSANLEKIITIGLFFSNFERNPPENTFLRLTAMATHSESNLSCFAQEDIAICRPHLAIKMPEKAEQYQPLTASVSLQNSLDAPMEDCVISILGRGLIHRERSYRFRSVWPENTMCAKFQFTPTHVGLQRLTVEVDCNMFQNLTNYKSVTVVAPELSA

• Full Name: erythrocyte membrane protein band 4.2
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC096093
• Gene Symbol: EPB42
• Gene ID (NCBI): 2038
• RRID: AB_3085962
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P16452
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EPB42, also known as Protein 4.2, is amongst the most abundant protein components of the erythrocyte membrane, representing approximately 5% membrane content or ~2.5 × 105 copies/cell. EPB42 binds to the cytoplasmic domain of band 3, which serves as a site for the anchorage of several membrane skeletal proteins(PMID: 19269200, 12049649). EPB42 is a 72 kDa peripheral membrane protein.