|Positive WB detected in||A431 cells, MCF-7 cells, mouse colon tissue|
|Positive IHC detected in||human breast cancer tissue, human colon cancer tissue, human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells, human colon cancer tissue|
|Positive FC detected in||A431 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21050-1-AP is EPCAM Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||EPCAM fusion protein Ag15393|
|Full Name||epithelial cell adhesion molecule|
|Calculated molecular weight||314 aa, 35 kDa|
|Observed molecular weight||35-40 kDa|
|GenBank accession number||BC014785|
|Gene ID (NCBI)||4072|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)
|Product Specific Protocols|
|WB protocol for EPCAM antibody 21050-1-AP||Download protocol|
|IHC protocol for EPCAM antibody 21050-1-AP||Download protocol|
|IF protocol for EPCAM antibody 21050-1-AP||Download protocol|
|FC protocol for EPCAM antibody 21050-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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