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EPCAM/CD326 Polyclonal antibody

EPCAM Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (1)





Cat no : 21050-1-AP


CD326, CO 17A, CO17 1A, EGP, EGP 2, EGP314, EGP34, EGP40, Ep CAM, EPCAM, Epithelial glycoprotein, Epithelial glycoprotein 314, ESA, GA733 2, hEGP 2, hEGP314, KS 1/4 antigen, KS1/4, KSA, M1S2, M4S1, MIC18, MK 1, TACST 1, TACSTD1, TROP1

Tested Applications

Positive WB detected inA431 cells, MCF-7 cells, mouse colon tissue
Positive IHC detected inhuman colon tissue, human colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, human colon cancer tissue
Positive FC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy EPCAM Ready-To-Use IHC Kit. EPCAM primary antibody included.

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:1200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21050-1-AP is EPCAM Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EPCAM fusion protein Ag15393
Full Name epithelial cell adhesion molecule
Calculated molecular weight 314 aa, 35 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC014785
Gene symbol EPCAM
Gene ID (NCBI) 4072
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)


Product Specific Protocols
WB protocol for EPCAM antibody 21050-1-APDownload protocol
IHC protocol for EPCAM antibody 21050-1-APDownload protocol
IF protocol for EPCAM antibody 21050-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Commun Biol

Hepatocyte growth factor activator inhibitor-2 stabilizes Epcam and maintains epithelial organization in the mouse intestine.

Authors - Makiko Kawaguchi

J Exp Clin Cancer Res

Tobacco smoke induced hepatic cancer stem cell-like properties through IL-33/p38 pathway.

Authors - Chunfeng Xie

Am J Transl Res

High CCL7 expression is associated with migration, invasion and bone metastasis of non-small cell lung cancer cells.

Authors - Shuai Han

Breast Cancer Res

MiR-7 reduces the BCSC subset by inhibiting XIST to modulate the miR-92b/Slug/ESA axis and inhibit tumor growth.

Authors - Miao Li

J Exp Clin Cancer Res

PMID: 31992334

Authors - Siqi Wang

Int J Mol Sci

Expression of Cancer Stem Cell Markers EpCAM and CD90 Is Correlated with Anti- and Pro-Oncogenic EphA2 Signaling in Hepatocellular Carcinoma.

Authors - Nobuhiko Asakura