EPCAM/CD326 Polyclonal antibody

• Featured Product
• KD/KO Validated

EPCAM/CD326 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, FC, chIP, ELISA

Conjugate

Unconjugated

Publications

52

Cat no : 21050-1-AP

Synonyms

CD326, CO 17A, CO17 1A, EGP, EGP 2, EGP314, EGP34, EGP40, Ep CAM, EPCAM, Epithelial glycoprotein, Epithelial glycoprotein 314, ESA, GA733 2, hEGP 2, hEGP314, KS 1/4 antigen, KS1/4, KSA, M1S2, M4S1, MIC18, MK 1, TACST 1, TACSTD1, TROP1

---

Validation Data Gallery

• 

  WB analysis of A431 using 21050-1-AP

  WB result of EPCAM antibody (21050-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPCAM transfected A431 cells.

• 

  WB analysis of A431 using 21050-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 21050-1-AP (EPCAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21050-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21050-1-AP (EPCAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 21050-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 21050-1-AP (EPCAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21050-1-AP (EPCAM antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21050-1-AP (EPCAM antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21050-1-AP (EPCAM Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21050-1-AP (EPCAM Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse colon using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 21050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 21050-1-AP (EPCAM antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human colon cancer using 21050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using 21050-1-AP (EPCAM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human colon cancer using 21050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using 21050-1-AP (EPCAM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 21050-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using EPCAM antibody (21050-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HT-29 using 21050-1-AP

  1X10^6 HT-29 cells were surface stained with 0.5 ug Anti-Human EPCAM/CD326 (21050-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were not fixed.

• 

  FC experiment of A431 using 21050-1-AP

  1X10^6 A431 cells were surface stained with 0.4 ug Anti-Human EPCAM (21050-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were not fixed.

• 

  FC experiment of HepG2 using 21050-1-AP

  1X10^6 HepG2 cells were surface stained with 0.4 ug Anti-Human EPCAM/CD326 (21050-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were not fixed.

---

Tested Applications

• Positive WB detected in: A431 cells, MCF-7 cells, mouse colon tissue
• Positive IHC detected in: human colon cancer tissue, mouse colon tissue, human breast cancer tissue, human colon tissue, mouse stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells, human colon cancer tissue
• Positive FC detected in: HT-29 cells, HepG2 cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 32 publications below
• IHC: See 12 publications below
• IF: See 14 publications below
• FC: See 2 publications below
• ChIP: See 1 publications below

Product Information

21050-1-AP targets EPCAM/CD326 in WB, IHC, IF, FC, chIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EPCAM/CD326 fusion protein Ag15393
• Full Name: epithelial cell adhesion molecule
• Calculated molecular weight: 314 aa, 35 kDa
• Observed molecular weight: 35-40 kDa
• GenBank accession number: BC014785
• Gene symbol: EPCAM
• Gene ID (NCBI): 4072
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)

Publications

Species	Application	Title
human	IF	Mol Cancer; m6A methylation reader IGF2BP2 activates endothelial cells to promote angiogenesis and metastasis of lung adenocarcinoma; Authors - Han Fang
mouse	IHC	Cancer Cell; Loss of Lkb1 and Pten leads to lung squamous cell carcinoma with elevated PD-L1 expression.; Authors - Chunxiao Xu
mouse	IF	Gut; Cross-talk between the gut microbiota and monocyte-like macrophages mediates an inflammatory response to promote colitis-associated tumourigenesis.; Authors - Yunben Yang
human	IF	Nat Commun; Single-cell RNA sequencing highlights the role of inflammatory cancer-associated fibroblasts in bladder urothelial carcinoma.; Authors - Zhaohui Chen
human	WB	Biosens Bioelectron; Tumor-cell detection, labeling and phenotyping with an electron-doped bifunctional signal-amplifier.; Authors - Hao Lu
human	WB	EMBO J; A stress-induced tyrosine-tRNA depletion response mediates codon-based translational repression and growth suppression.; Authors - Doowon Huh

Related Products

EPCAM/CD326 Monoclonal antibody

66316-1-Ig

E-cadherin Polyclonal antibody

20874-1-AP

E-cadherin Monoclonal antibody

60335-1-Ig

P53 Polyclonal antibody

10442-1-AP

FABP5 Polyclonal antibody

12348-1-AP

c-MYC Polyclonal antibody

10828-1-AP

c-Myc Polyclonal antibody

10057-1-AP

Cyclin D1 Monoclonal antibody

60186-1-Ig