CoraLite® Plus 488-conjugated EPCAM Polyclonal antibody

• Featured Product
• KD/KO Validated

EPCAM Polyclonal Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-21050

Synonyms

CD326, CO 17A, CO17 1A, EGP, EGP 2, EGP314, EGP34, EGP40, Ep CAM, EPCAM, Epithelial glycoprotein, Epithelial glycoprotein 314, ESA, GA733 2, hEGP 2, hEGP314, KS 1/4 antigen, KS1/4, KSA, M1S2, M4S1, MIC18, MK 1, TACST 1, TACSTD1, TROP1

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Validation Data Gallery

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  FC experiment of MCF-7 using CL488-21050

  1X10^6 MCF-7 cells were surface stained with 0.4 ug CoraLite® Plus 488 Anti-Human EPCAM (CL488-21050) (red), or 0.4 ug Control Antibody. Cells were not fixed.

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  FC experiment of HT-29 using CL488-21050

  1X10^6 HT-29 cells were surface stained with 0.4 ug CoraLite® Plus 488 Anti-Human EPCAM (CL488-21050) (red), or 0.4 ug Control Antibody. Cells were not fixed.

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Tested Applications

• Positive FC detected in: MCF-7 cells, HT-29 cells

Recommended dilution

Application	Dilution
Sample-dependent, check data in validation data gallery

Product Information

CL488-21050 targets EPCAM in FC applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EPCAM fusion protein Ag15393
• Full Name: epithelial cell adhesion molecule
• Calculated molecular weight: 314 aa, 35 kDa
• Observed molecular weight: 35-40 kDa
• GenBank accession number: BC014785
• Gene symbol: EPCAM
• Gene ID (NCBI): 4072
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)