EPHA2 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 87562-1-RR (same clone as 87562-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87562-1-RR (EPHA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87562-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 87562-1-RR (same clone as 87562-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using EPHA2 antibody (87562-1-RR, Clone: 252900E11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87562-1-PBS in a different storage buffer formulation.

EPHA2 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 87562-1-PBS

Clone No.252900E11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

EC:2.7.10.1, ECK, EPH receptor A2, Ephrin type A receptor 2, Ephrin type-A receptor 2

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Product Information

87562-1-PBS targets EPHA2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0146

  Product name: Recombinant human EPHA2 protein

  Source: mammalian cells-derived, pHZ-KIsec

  Tag: Myc & 6*His

  Domain: 24-534 aa of BC037166

  Sequence: AQGKEVVLLDFAAAGGELGWLTHPYGKGWDLMQNIMNDMPIYMYSVCNVMSGDQDNWLRTNWVYRGEAERIFIELKFTVRDCNSFPGGASSCKETFNLYYAESDLDYGTNFQKRLFTKIDTIAPDEITVSSDFEARHVKLNVEERSVGPLTRKGFYLAFQDIGACVALLSVRVYYKKCPELLQGLAHFPETIAGSDAPSLATVAGTCVDHAVVPPGGEEPRMHCAVDGEWLVPIGQCLCQAGYEKVEDACQACSPGFFKFEASESPCLECPEHTLPSPEGATSCECEEGFFRAPQDPASMPCTRPPSAPHYLTAVGMGAKVELRWTPPQDSGGREDIVYSVTCEQCWPESGECGPCEASVRYSEPPHGLTRTSVTVSDLEPHMNYTFTVEARNGVSGLVTSRSFRTASVSINQTEPPKVRLEGRSTTSLSVSWSIPPPQQSRVWKYEVTYRKKGDSNSYNVRRTEGFSVTLDDLAPDTTYLVQVQALTQEGQGAGSKVHEFQTLSPEGSGN

• Full Name: EPH receptor A2
• Calculated Molecular Weight: 976 aa, 108 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC037166
• Gene Symbol: EPHA2
• Gene ID (NCBI): 1969
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P29317
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

EPHA2 (Ephrin type-A receptor 2) belongs to the receptor tyrosine kinase (RTK) family, with 16 known receptors and 9 known membrane-bound ligands in all species. Based on the extracellular domain sequence homology, structure, and binding affinity, Eph receptors and their ephrin ligands can be divided into A and B subtypes (PMID: 33962882). EPHA2 contains a conserved N-terminal ligand-bound extracellular domain, a transmembrane domain, and a conserved tyrosine kinase domain (PMID: 8070404).