|Positive WB detected in||HEK-293T cells, PC-3 cells, HeLa cells, Calyculin A treated HEK-293T cells, Calyculin A treated PC-3 cells, Calyculin A treated HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Sample-dependent, check data in validation data gallery|
80031-1-RR targets Phospho-ERK1/2 (Thr202/Tyr204) in WB applications and shows reactivity with Human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||mitogen-activated protein kinase 3|
|Calculated molecular weight||38-43 kDa|
|Observed molecular weight||38-40 kDa|
|GenBank accession number||NM_002746|
|Gene ID (NCBI)||5595|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1.
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