Phospho-ERK1/2 (Thr202/Tyr204) Recombinant monoclonal antibody

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  WB analysis using 80031-1-RR

  Non-treated HEK-293T and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 80031-1-RR (Phospho-ERK1/2 (Thr202/Tyr204) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 80031-1-RR

  Non-treated HeLa and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 80031-1-RR (Phospho-ERK1/2 (Thr202/Tyr204) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 80031-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80031-1-RR (Phospho-ERK1/2 (Thr202/Tyr204) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 80031-1-RR

  Non-treated PC-3 and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 80031-1-RR (Phospho-ERK1/2 (Thr202/Tyr204) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 80031-1-RR

  Non-treated HeLa and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 80031-1-RR (ERK1/2-phospho-Tyr204 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of HepG2 using 80031-1-RR

  1X10^6 HepG2 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.06 ug Phospho-ERK1/2 (Thr202/Tyr204) Recombinant antibody (80031-1-RR, Clone:8D12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Phospho-ERK1/2 (Thr202/Tyr204) Uni-rAb^® Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 80031-1-RR

Clone No.8D12

Publications(48)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, FC (Intra), ELISA

Synonyms

ERK (Thr202/Tyr204), ERK1/2 (Thr202/Tyr204), ERK2 (Thr185/Tyr187), p ERK, p ERK (Thr202/Tyr204)

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, Calyculin A treated NIH/3T3 cells, PC-3 cells, Calyculin A treated HeLa cells, Calyculin A treated PC-3 cells
• Positive FC (Intra) detected in: Calyculin A treated HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 47 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

80031-1-RR targets Phospho-ERK1/2 (Thr202/Tyr204) in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: mitogen-activated protein kinase 3
• Calculated Molecular Weight: 38-43 kDa
• Observed Molecular Weight: 38-40 kDa
• GenBank Accession Number: NM_002746
• Gene Symbol: ERK1
• Gene ID (NCBI): 5595
• RRID: AB_2918858
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P27361
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. This antibody simultaneously recognizes the phosphorylation of ERK1 at Thr202 and Tyr204, as well as the phosphorylation of ERK2 at Thr185 and Tyr187.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Adv Sci (Weinh); Ccl2-Induced Regulatory T Cells Balance Inflammation Through Macrophage Polarization During Liver Reconstitution; Authors - Rui Wang
human	WB	Br J Pharmacol; Allicin ameliorates imiquimod-induced psoriasis-like skin inflammation via disturbing the interaction of keratinocytes with IL-17A; Authors - Lu Zhang
human	WB	Cell Death Dis; USP4 promotes the proliferation, migration, and invasion of esophageal squamous cell carcinoma by targeting TAK1; Authors - Hongdian Zhang
rat	WB	Cell Commun Signal; Spinster homolog 2/S1P signaling ameliorates macrophage inflammatory response to bacterial infections by balancing PGE2 production; Authors - Chao Fang
mouse	WB	Aging (Albany NY); METTL3 regulates hippocampal gene transcription via N6-methyladenosine methylation in sevoflurane-induced postoperative cognitive dysfunction mouse; Authors - Baiqing He
human	WB	J Cell Physiol; PKC-β/Alox5 axis activation promotes Bcr-Abl-independent TKI-resistance in chronic myeloid leukemia; Authors - Dan Ma

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (09-30-2025)

phospho-ERK (in green) worked good enough although weakly in human brain FFPE tissue

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

Phospho-ERK1/2 (Thr202/Tyr204) Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:80031-1-RR)