ERO1L Polyclonal antibody

ERO1L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 12007-1-AP

Synonyms

ERO1 alpha, ERO1 L, ERO1 L alpha, ERO1 like (S. cerevisiae), ERO1 like protein alpha, ERO1L, Oxidoreductin 1 L alpha

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Validation Data Gallery

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  WB analysis of mouse ovary using 12007-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12007-1-AP (ERO1L antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 12007-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12007-1-AP (ERO1L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 12007-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12007-1-AP (ERO1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12007-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12007-1-AP (ERO1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12007-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12007-1-AP (ERO1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12007-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12007-1-AP (ERO1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse ovary tissue, A431 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

12007-1-AP targets ERO1L in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ERO1L fusion protein Ag2620
• Full Name: ERO1-like (S. cerevisiae)
• Calculated Molecular Weight: 468 aa, 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC008674
• Gene Symbol: ERO1L
• Gene ID (NCBI): 30001
• RRID: AB_10666441
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERO1L, also named as ERO1-alpha, is an essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. It acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. It does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Glutathione may be required to regulate its activity in the endoplasmic reticulum. It may be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. It is required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin. This antibody has no cross reaction to ERO1.

Publications

Species	Application	Title
human	WB	Cancers (Basel); ER Stress Response and Induction of Apoptosis in Malignant Pleural Mesothelioma: The Achilles Heel Targeted by the Anticancer Ruthenium Drug BOLD-100; Authors - Elia Ranzato
mouse	WB	Cell Calcium; Mannan-binding lectin deficiency augments hepatic endoplasmic reticulum stress through IP3R-controlled calcium release.; Authors - Yu Chen
pig	WB	J Virol; Porcine circovirus 2 manipulates PERK-ERO1α axis of endoplasmic reticulum in favor of its replication by derepressing viral DNA from HMGB1 sequestration within nuclei.; Authors - Renjie Sun
mouse	WB	J Toxicol; Oxidative Stress and the ER Stress Response in a Murine Model for Early-Stage Alcoholic Liver Disease.; Authors - Galligan James J JJ
mouse	WB,IF	Brain Res; Role of Ero1α in cognitive impairment induced by chronic hypoxia; Authors - Nan Zhang
rat	WB	Transplantation; Protective Effect of Calpain Inhibition During Cold Ischemia on Ischemia-reperfusion Injury After Lung Transplantation; Authors - Yuuki Matsui