ERp72 Monoclonal antibody

ERp72 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1


human and More (1)







Cat no : 66365-1-Ig


Cell and organelle markers, endoplasmic reticulum marker, ER marker, ER protein 70, ER protein 72, ERp 72, ERP70, ERp72, PDIA4, Protein disulfide isomerase A4

Tested Applications

Positive WB detected inA431 cells, JAR cells, HEK-293 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66365-1-Ig targets ERp72 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ERp72 fusion protein Ag6873
Full Name protein disulfide isomerase family A, member 4
Calculated Molecular Weight 73 kDa
Observed Molecular Weight 72 kDa
GenBank Accession NumberBC000425
Gene Symbol PDIA4
Gene ID (NCBI) 9601
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDIA4 (Protein disulfide-isomerase A4) is also named ERP70, ERP72, and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of -S-S- bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal, KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif, CGHC(PMID:15475357). The full-length protein has a signal peptide with 20 amino acids.


Product Specific Protocols
WB protocol for ERp72 antibody 66365-1-IgDownload protocol
IHC protocol for ERp72 antibody 66365-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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