ERp72 Monoclonal antibody, PBS Only

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  WB analysis using 66365-1-Ig (same clone as 66365-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66365-1-Ig (ERp72 antibody) at dilution of 1:4900 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66365-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66365-1-Ig (same clone as 66365-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66365-1-Ig (ERp72 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66365-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66365-1-Ig (same clone as 66365-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66365-1-Ig (ERp72 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66365-1-PBS in a different storage buffer formulation.

ERp72 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 66365-1-PBS

Clone No.1D5F3

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Cell and organelle markers, endoplasmic reticulum marker, ER marker, ER protein 70, ER protein 72, ERp 72, ERP70, ERp72, PDIA4, Protein disulfide isomerase A4

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Product Information

66365-1-PBS targets ERp72 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6873

  Product name: Recombinant human ERp72 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 294-645 aa of BC000425

  Sequence: KQVQEFLKDGDDVIIIGVFKGESDPAYQQYQDAANNLREDYKFHHTFSTEIAKFLKVSQGQLVVMQPEKFQSKYEPRSHMMDVQGSTQDSAIKDFVLKYALPLVGHRKVSNDAKRYTRRPLVVVYYSVDFSFDYRAATQFWRSKVLEVAKDFPEYTFAIADEEDYAGEVKDLGLSESGEDVNAAILDESGKKFAMEPEEFDSDTLREFVTAFKKGKLKPVIKSQPVPKNNKGPVKVVVGKTFDSIVMDPKKDVLIEFYAPWCGHCKQLEPVYNSLAKKYKGQKGLVIAKMDATANDVPSDRYKVEGFPTIYFAPSGDKKNPVKFEGGDRDLEHLSKFIEEHATKLSRTKEEL

• Full Name: protein disulfide isomerase family A, member 4
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC000425
• Gene Symbol: ERp72
• Gene ID (NCBI): 9601
• RRID: AB_2881745
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P13667
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PDIA4 (Protein disulfide-isomerase A4) is also named ERP70, ERP72, and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of -S-S- bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal, KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif, CGHC(PMID:15475357). The full-length protein has a signal peptide with 20 amino acids.