ESPL1 Polyclonal antibody

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  WB analysis using 22261-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22261-1-AP (ESPL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

ESPL1 Polyclonal Antibody for WB, ELISA

Cat No. 22261-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Caspase like protein ESPL1, ESP1, KIAA0165, SEPARASE, SEPARIN

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Tested Applications

• Positive WB detected in: A431 cells, Jurkat cells, Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22261-1-AP targets ESPL1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: extra spindle pole bodies homolog 1 (S. cerevisiae)
• Calculated Molecular Weight: 233 kDa
• Observed Molecular Weight: 170 kDa
• GenBank Accession Number: NM_012291
• Gene Symbol: ESPL1
• Gene ID (NCBI): 9700
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14674
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ESPL1 (Extra spindle poles-like 1 protein), also known as Separin, Separase, a cysteine endopeptidase, plays a vital role in the stable binding between sister chromatids before anaphase and their timely separation during anaphase, which is the key to chromosome inheritance (PMID: 34512713). ESPL1 expression is elevated and predicts poor outcomes in patients with various types of cancer, including breast cancer, glioma and endometrial cancer (PMID: 36465268). Full-length ESPL1 decreased after 30 min resulting in a ~ 170 kDa N-terminal (ESPL1N−term) and a ~ 60 kDa C-terminal polypeptide (ESPL1CC−term), reflecting ESPL1 activation and auto-cleavage (PMID: 30429481)