EXOSC4 Polyclonal antibody

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  WB analysis using 15937-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15937-1-AP (EXOSC4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15937-1-AP

  WB result of EXOSC4 antibody (15937-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC4 transfected Jurkat cells.

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  IHC staining of human skin cancer using 15937-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15937-1-AP (EXOSC4 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 15937-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15937-1-AP (EXOSC4 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 15937-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15937-1-AP (EXOSC4 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 15937-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15937-1-AP (EXOSC4 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 15937-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EXOSC4 antibody (15937-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 15937-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EXOSC4 antibody (15937-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

EXOSC4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15937-1-AP

Publications(4)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SKI6, RRP41, Ribosomal RNA-processing protein 41, p12A, Exosome complex component RRP41

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Tested Applications

• Positive WB detected in: HEK-293T cells, Jurkat cells, HeLa cells, SW480 cells, HCT 116 cells, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

15937-1-AP targets EXOSC4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, yeast
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EXOSC4 fusion protein Ag7595
• Full Name: exosome component 4
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC002777
• Gene Symbol: EXOSC4
• Gene ID (NCBI): 54512
• RRID: AB_2293806
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NPD3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EXOSC4, also named as RRP41 and SKI6, belongs to the RNase PH family. EXOSC4 is a novel component of the human exosome complex. EXOSC4 is the most important RNA processing machinery in maturation and degradation of RNA playing a major role in gene expression regulation (PMID: 29626590). EXOSC4 has 1 isoform with the molecular mass of 27 kDa. However, studies have shown that EXOSC4 can interact with EXOSC2 and EXOSC7, possibly form a complex with the molecular weight of 90 kDa (PMID: 25115889). Recently, EXOSC4 has been found to play important roles in tumorigcncsis and development including CRC (PMID: 33075373).

Publications

Species	Application	Title
human	WB	J Biol Chem; A Point Mutation in the Exon Junction Complex Factor Y14 Disrupts Its Function in mRNA Cap Binding and Translation Enhancement.; Authors - Tzu-Wei Chuang
human	WB,IHC	Life Sci; STX2 drives colorectal cancer proliferation via upregulation of EXOSC4.; Authors - Yong-Xia Wang ; KD Validated
human	WB	Life Sci Alliance; Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication; Authors - Tobias Moll
yeast	WB	G3 (Bethesda); Humanized Saccharomyces cerevisiae provides a facile and effective tool to identify damaging human variants that cause exosomopathies; Authors - Khondakar Sayef Ahammed

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tsimafei (Verified Customer) (07-06-2022)

Control and Exosc4 depletion shows a reduction of Exosc4 signal. Antibodies were incubated ON at 4C.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mESCs

EXOSC4 Antibody Western Blot validation (1:2000 dilution) in mESCs (Cat no:15937-1-AP)