|Positive WB detected in||HEK-293T cells, human liver tissue, human heart tissue, HeLa cells, Jurkat cells, SW480 cels, HCT 116 cells|
|Positive IHC detected in||human skin cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
15937-1-AP targets EXOSC4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||EXOSC4 fusion protein Ag7595|
|Full Name||exosome component 4|
|Calculated molecular weight||26 kDa|
|Observed molecular weight||27 kDa|
|GenBank accession number||BC002777|
|Gene ID (NCBI)||54512|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
EXOSC4, also named as RRP41 and SKI6, belongs to the RNase PH family. EXOSC4 is a novel component of the human exosome complex. EXOSC4 is the most important RNA processing machinery in maturation and degradation of RNA playing a major role in gene expression regulation (PMID: 29626590). EXOSC4 has 1 isoform with the molecular mass of 27 kDa. Recently, EXOSC4 has been found to play important roles in tumorigcncsis and development including CRC (PMID: 33075373).
STX2 drives colorectal cancer proliferation via upregulation of EXOSC4.
J Biol Chem
A Point Mutation in the Exon Junction Complex Factor Y14 Disrupts Its Function in mRNA Cap Binding and Translation Enhancement.