EXOSC4 Polyclonal antibody

EXOSC4 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15937-1-AP

Synonyms

EXOSC4, exosome component 4, hRrp41p, p12A, RRP41, RRP41A, Rrp41p, SKI6, Ski6p



Tested Applications

Positive WB detected inHEK-293T cells, human liver tissue, human heart tissue, HeLa cells, Jurkat cells, SW480 cels, HCT 116 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15937-1-AP targets EXOSC4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EXOSC4 fusion protein Ag7595
Full Name exosome component 4
Calculated molecular weight 26 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC002777
Gene symbol EXOSC4
Gene ID (NCBI) 54512
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EXOSC4, also named as RRP41 and SKI6, belongs to the RNase PH family. EXOSC4 is a novel component of the human exosome complex. EXOSC4 is the most important RNA processing machinery in maturation and degradation of RNA playing a major role in gene expression regulation (PMID: 29626590). EXOSC4 has 1 isoform with the molecular mass of 27 kDa. Recently, EXOSC4 has been found to play important roles in tumorigcncsis and development including CRC (PMID: 33075373).

Protocols

Product Specific Protocols
WB protocol for EXOSC4 antibody 15937-1-APDownload protocol
IHC protocol for EXOSC4 antibody 15937-1-APDownload protocol
IF protocol for EXOSC4 antibody 15937-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Life Sci

STX2 drives colorectal cancer proliferation via upregulation of EXOSC4.

Authors - Yong-Xia Wang
  • KD Validated
humanWB

J Biol Chem

A Point Mutation in the Exon Junction Complex Factor Y14 Disrupts Its Function in mRNA Cap Binding and Translation Enhancement.

Authors - Tzu-Wei Chuang