Endoglin/CD105 Monoclonal antibody

Endoglin/CD105 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2D5E8

Cat no : 67075-1-Ig

Synonyms

CD105, END, endoglin, ENG, FLJ41744, HHT1, ORW, ORW1



Tested Applications

Positive WB detected inHL-60 cells, HUVEC cells, human placenta tissue
Positive IHC detected inhuman breast cancer tissue, human placenta tissue, human renal cell carcinoma tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue, human liver cancer tissue
Positive FC detected inHUVEC cells
Planning an IHC experiment? We recommend our IHCeasy CD105 Ready-To-Use IHC Kit. CD105 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:10000-1:40000
Immunofluorescence (IF)IF : 1:5000-1:20000
Sample-dependent, check data in validation data gallery

Product Information

67075-1-Ig targets Endoglin/CD105 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Endoglin/CD105 fusion protein Ag27837
Full Name endoglin
Calculated molecular weight 71 kDa
Observed molecular weight 95-100 kDa
GenBank accession numberBC014271
Gene symbol ENG
Gene ID (NCBI) 2022
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

Protocols

Product Specific Protocols
WB protocol for Endoglin/CD105 antibody 67075-1-IgDownload protocol
IHC protocol for Endoglin/CD105 antibody 67075-1-IgDownload protocol
IF protocol for Endoglin/CD105 antibody 67075-1-IgDownload protocol
FC protocol for Endoglin/CD105 antibody 67075-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols