Endoglin/CD105 Monoclonal antibody, PBS Only

Endoglin/CD105 Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D5E8

Cat no : 67075-1-PBS

Synonyms

CD105, END, endoglin, ENG, FLJ41744, HHT1, ORW, ORW1

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Validation Data Gallery

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  WB analysis using 67075-1-Ig (same clone as 67075-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HL-60 using 67075-1-Ig (same clone as 67075-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HUVEC using 67075-1-Ig (same clone as 67075-1-PBS)

  HUVEC cells were subjected to SDS PAGE followed by western blot with 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 67075-1-Ig (same clone as 67075-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67075-1-Ig (same clone as 67075-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:20000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67075-1-Ig (same clone as 67075-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:20000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 67075-1-Ig (same clone as 67075-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:20000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 67075-1-Ig (same clone as 67075-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:20000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 67075-1-Ig (same clone as 67075-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:20000 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 67075-1-Ig (same clone as 67075-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:20000 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67075-1-Ig (same clone as 67075-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67075-1-Ig (same clone as 67075-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 67075-1-Ig (same clone as 67075-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Endoglin/CD105 antibody (67075-1-Ig, Clone: 2D5E8 ) at dilution of 1:10000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 67075-1-Ig (same clone as 67075-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Endoglin/CD105 antibody (67075-1-Ig, Clone: 2D5E8 ) at dilution of 1:10000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 67075-1-Ig (same clone as 67075-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Endoglin/CD105 antibody (67075-1-Ig, Clone: 2D5E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 67075-1-Ig (same clone as 67075-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Endoglin/CD105 antibody (67075-1-Ig, Clone: 2D5E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HUVEC using 67075-1-Ig (same clone as 67075-1-PBS)

  1X10^6 HUVEC cells were intracellularly stained with 0.25 ug Anti-Human Endoglin/CD105 (67075-1-Ig, Clone:2D5E8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.25 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67075-1-PBS targets Endoglin/CD105 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Endoglin/CD105 fusion protein Ag27837
• Full Name: endoglin
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 95-100 kDa
• GenBank Accession Number: BC014271
• Gene Symbol: ENG
• Gene ID (NCBI): 2022
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)