CoraLite® Plus 647-conjugated Endoglin/CD105 Monoclonal antibody

Endoglin/CD105 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2D5E8

Cat no : CL647-67075

Synonyms

CD105, END, endoglin, ENG, FLJ41744, HHT1, ORW, ORW1



Tested Applications

Positive IF detected inhuman tonsillitis tissue
Positive FC detected inHUVEC cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-67075 targets Endoglin/CD105 in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Endoglin/CD105 fusion protein Ag27837
Full Name endoglin
Calculated Molecular Weight 71 kDa
Observed Molecular Weight 95-100 kDa
GenBank Accession NumberBC014271
Gene Symbol ENG
Gene ID (NCBI) 2022
RRIDAB_2920290
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 Endoglin/CD105 antibody CL647-67075Download protocol
FC protocol for CL Plus 647 Endoglin/CD105 antibody CL647-67075Download protocol
Standard Protocols
Click here to view our Standard Protocols