Ezrin Polyclonal antibody

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  WB analysis of HeLa using 26056-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26056-1-AP (Ezrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26056-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26056-1-AP (Ezrin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 26056-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 26056-1-AP (Ezrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 26056-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26056-1-AP (Ezrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 26056-1-AP

  IP result of anti-Ezrin (IP:26056-1-AP, 4ug; Detection:26056-1-AP 1:2000) with HeLa cells lysate 3200ug.

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  IHC staining of mouse stomach using 26056-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 26056-1-AP (Ezrin antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 26056-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 26056-1-AP (Ezrin antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 26056-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 26056-1-AP (Ezrin antibody) at dilution of 1:8000 (under 40x lens).

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  IHC staining of human small intestine using 26056-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 26056-1-AP (Ezrin antibody) at dilution of 1:2000 (under 10x lens).

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  IHC staining of human small intestine using 26056-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 26056-1-AP (Ezrin antibody) at dilution of 1:2000 (under 40x lens).

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  IF Staining of mouse eye using 26056-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Ezrin antibody (26056-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse eye using 26056-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Ezrin antibody (26056-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat eye using 26056-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat eye tissue using Ezrin antibody (26056-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 26056-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Ezrin antibody (26056-1-AP) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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  FC experiment of HeLa using 26056-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug Ezrin Polyclonal antibody (26056-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

Ezrin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Cat No. 26056-1-AP

Publications(16)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, RIP, ELISA

Synonyms

EZR, VIL2, Villin-2

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Tested Applications

• Positive WB detected in: HeLa cells, mouse kidney tissue, NIH/3T3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse stomach tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: rat eye tissue, mouse eye tissue
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Product Information

26056-1-AP targets Ezrin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Ezrin fusion protein Ag23530
• Full Name: ezrin
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC013903
• Gene Symbol: Ezrin
• Gene ID (NCBI): 7430
• ENSEMBL Gene ID: ENSG00000092820
• RRID: AB_2722561
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P15311
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ezrin is a member of the ERM (Ezrin, Radixin, Moesin) protein family and serves as a crucial linker between the cell membrane and the actin cytoskeleton. It plays significant roles in various cellular processes, including maintaining cell morphology, facilitating cell movement and adhesion, regulating signal transduction, and influencing apoptosis.

Publications

Species	Application	Title
mouse,human	IF	Acta Neuropathol; Selective vulnerability of tripartite synapses in amyotrophic lateral sclerosis.; Authors - Matthew J Broadhead
human	WB	Proc Natl Acad Sci U S A; Cotranslational interaction of human EBP50 and ezrin overcomes masked binding site during complex assembly.; Authors - Krishnendu Khan ; KO Validated
mouse	WB	Elife; Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility.; Authors - Dao-Lai Zhang
human	WB	Int J Mol Sci; Bidirectional Tumor-Promoting Activities of Macrophage Ezrin.; Authors - Krishnendu Khan ; KD Validated
mouse	WB	J Biol Chem; Ciliary neurotrophic factor (CNTF) protects retinal cone and rod photoreceptors by suppressing excessive formation of the visual pigments.; Authors - Songhua Li
pig	WB,IF	J Virol; Pemetrexed alleviates piglet diarrhea by blocking the interaction between porcine epidemic diarrhea virus nucleocapsid protein and Ezrin; Authors - Shujuan Zhang