F2 Monoclonal antibody

F2 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1E12A8

Cat no : 66509-1-Ig

Synonyms

Coagulation factor II, F2, Prothrombin, PT



Tested Applications

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66509-1-Ig targets F2 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen F2 fusion protein Ag18385
Full Name coagulation factor II (thrombin)
Calculated molecular weight 622 aa, 70 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC051332
Gene symbol F2
Gene ID (NCBI) 2147
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Factor II, or prothrombin, is one of the components of this chain of plasma proteins involved in blood coagulation. Thrombin is essential in the processes of hemostasis and thrombosis.

Protocols

Product Specific Protocols
WB protocol for F2 antibody 66509-1-IgDownload protocol
IHC protocol for F2 antibody 66509-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols