Tested Applications
| Positive WB detected in | unboiled J774A.1 cells, unboiled RAW 264.7 cells |
| Positive IHC detected in | mouse spleen tissue, rat spleen tissue, rat liver tissue, mouse lung tissue, mouse heart tissue, mouse brain tissue, mouse liver tissue, mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse spleen tissue, mouse liver tissue |
| Positive IF-Fro detected in | mouse spleen tissue |
| Positive IF/ICC detected in | RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:4000-1:16000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28463-1-AP targets F4/80 in WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, duck |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28252 Product name: Recombinant mouse Adgre1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 443-644 aa of NM_010130 Sequence: MELSKEETSTLGTILLETVESTMLAALLIPSGNASQMIQTEYLDIESKVINEECKENESINLAARGDKMNVGCFIIKESVSTGAPGVAFVSFAHMESVLNERFFEDGQSFRKLRMNSRVVGGTVTGEKKEDFSKPIIYTLQHIQPKQKSERPICVSWNTDVEDGRWTPSGCEIVEASETHTVCSCNRMANLAIIMASGELTME Predict reactive species |
| Full Name | EGF-like module containing, mucin-like, hormone receptor-like sequence 1 |
| Calculated Molecular Weight | 102 kDa |
| Observed Molecular Weight | 130 kDa, 160 kDa |
| GenBank Accession Number | NM_010130 |
| Gene Symbol | F4/80 |
| Gene ID (NCBI) | 13733 |
| RRID | AB_2881149 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q61549 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Mouse F4/80, also named as EMR1 and Gpf480, is a 160-kDa cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for F4/80 antibody 28463-1-AP | Download protocol |
| IHC protocol for F4/80 antibody 28463-1-AP | Download protocol |
| WB protocol for F4/80 antibody 28463-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The F4/80 polyclonal antibody (Cat# 28463-1-AP) has 518 total citations. Publications appear in high-impact journals including Nature Communications, Nature Immunology, Advanced Materials, Bioactive Materials, ACS Nano, Science Advances, Journal of Clinical Investigation, Cell Reports, and Oncogene, among many others. Research fields span macrophage biology and polarization, inflammation, cancer immunology, liver disease (NAFLD/NASH), kidney injury, cardiovascular disease, ferroptosis/pyroptosis, nanomedicine, wound healing, and metabolic disorders.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Porphyromonas gingivalis aggravates atherosclerotic plaque instability by promoting lipid-laden macrophage necroptosis | ||
Adv Mater Fully Biocompatible Tantalum-Based Antioxidant Nanoshields for Proximal Tubule Epithelial Cells-Targeted Mitochondrial Holistic Protection in Acute Kidney Injury. | ||
Adv Mater Efficient Therapy of Inflammatory Bowel Disease (IBD) with Highly Specific and Durable Targeted Ta2 C Modified with Chondroitin Sulfate (TACS) | ||
Nat Immunol Dermal macrophages set pain sensitivity by modulating the amount of tissue NGF through an SNX25-Nrf2 pathway | ||
Gut High-protein diets alleviate tumour growth and drug resistance by promoting AKT aggregation and turnover |
Reviews
What customers say
Users report mixed results depending on tissue type. One reviewer (IHC, mouse tissues) found strong staining in liver, spleen, and lung but poor performance on heart even after testing dilutions from 1:500 to 1:8000, rating it 3/5. Another reviewer achieved excellent results (5/5) using immunofluorescence on mouse uterus at 1:100 dilution with EDTA pH9 antigen retrieval and overnight primary antibody incubation at 4°C. Overall, the antibody performs well in most tissues but may require optimization for certain applications like cardiac tissue.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Lucia (Verified Customer) (04-08-2026) | Worked perfectly on liver, spleen and lung, but could not get a good staining on the heart. Tried multiple concentrations from 1:500 to 1:8000.
|
Kannan (Verified Customer) (06-13-2023) | AR using EDTApH9. overnight incubation of primary antibody at 4c.
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