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FAAH Polyclonal antibody

FAAH Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 17909-1-AP

Publications (10)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

Anandamide amidohydrolase 1, FAAH, FAAH 1, FAAH1, fatty acid amide hydrolase, Fatty acid amide hydrolase 1, Oleamide hydrolase 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIPIHC

WB analysis using 17909-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 17909-1-AP (FAAH antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of A431 using 17909-1-AP
WB result of FAAH antibody (17909-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAAH transfected A431 cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of A431 using 17909-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 17909-1-AP (FAAH Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of rat testis using 17909-1-AP
rat testis tissue were subjected to SDS PAGE followed by western blot with 17909-1-AP (FAAH Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse liver using 17909-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 17909-1-AP (FAAH Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of mouse liver using 17909-1-AP
IP result of anti-FAAH (IP:17909-1-AP, 4ug; Detection:17909-1-AP 1:500) with mouse liver tissue lysate 6400 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse testis using 17909-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17909-1-AP (FAAH antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse testis using 17909-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17909-1-AP (FAAH antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 17909-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17909-1-AP (FAAH Antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 17909-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17909-1-AP (FAAH Antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 17909-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17909-1-AP (FAAH antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 17909-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17909-1-AP (FAAH antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (10)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	mouse liver tissue, rat testis tissue, A431 cells
Positive IP detected in	mouse liver tissue
Positive IHC detected in	mouse testis tissue, human placenta tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:5000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:300
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17909-1-AP targets FAAH in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag12304 Product name: Recombinant human FAAH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 236-579 aa of BC093632 Sequence: TDIGGSIRFPSSFCGICGLKPTGNRLSKSGLKGCVYGQEAVRLSVGPMARDVESLALCLRALLCEDMFRLDPTVPPLPFREEVYTSSQPLRVGYYETDNYTMPSPAMRRAVLETKQSLEAAGHTLVPFLPSNIPHALETLSTGGLFSDGGHTFLQNFKGDFVDPCLGDLVSILKLPQWLKGLLAFLVKPLLPRLSAFLSNMKSRSAGKLWELQHEIEVYRKTVIAQWRALDLDVVLTPMLAPALDLNAPGRATGAVSYTMLYNCLDFPAGVVPVTTVTAEDEAQMEHYRGYFGDIWDKMLQKGMKKSVGLPVAVQCVALPWQEELCLRFMREVERLMTPEKQSS Predict reactive species
Full Name	fatty acid amide hydrolase
Calculated Molecular Weight	579 aa, 63 kDa
Observed Molecular Weight	55-65 kDa
GenBank Accession Number	BC093632
Gene Symbol	FAAH
Gene ID (NCBI)	2166
RRID	AB_2878465
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O00519
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fatty acid amide hydrolase (FAAH) is a membrane-bound homodimeric enzyme that in vivo controls content and biological activity of N-arachidonoylethanolamine (AEA) and other relevant bioactive lipids termed endocannabinoids. (PMID: 32041998) This antibody is specific for FAAH protein.

Protocols

Product Specific Protocols
IHC protocol for FAAH antibody 17909-1-AP	Download protocol
IP protocol for FAAH antibody 17909-1-AP	Download protocol
WB protocol for FAAH antibody 17909-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Anti-FAAH (catalog #17909-1-AP) has 10 total citations published in journals including Cell Death Differentiation, Cell Death & Disease, eLife, Phytomedicine, Journal of Ethnopharmacology, Frontiers in Nutrition, BBA Molecular Basis of Disease, Journal of Proteome Research, and Neuroscience. Research fields span the endocannabinoid system, ferroptosis, renal fibrosis, neural impairment, platelet activation, arthritis metabolomics, steroidogenesis, and sleep-related memory deficits.

Species	Application	Title
human,mouse	WB,IP	Cell Death Differ FAAH served a key membrane-anchoring and stabilizing role for NLRP3 protein independently of the endocannabinoid system Authors - Yangyang Zhu KO Validated View Article
human	WB	Cell Death Dis Combination treatment with FAAH inhibitors/URB597 and ferroptosis inducers significantly decreases the growth and metastasis of renal cell carcinoma cells via the PI3K-AKT signaling pathway Authors - Junfeng Hao View Article
		Phytomedicine Schisandra chinensis lignans regulate and cooperate with endogenous cannabinoid systems to ameliorate intestinal barrier injury associated with depression Authors - Jinyu Wang View Article
human	WB	J Ethnopharmacol Qihuang Zhuyu Formula Modulates Mitochondrial Function to Inhibit Platelet Activation via Endocannabinoid Signaling Mediated by the SIRT1/PPARα Pathway Authors - Ruijie Shi View Article
rat	WB	Front Nutr Neonatal supplementation of oleamide during suckling ameliorates maternal postpartum sleep interruption-induced neural impairment and endocannabinoid dysfunction in early adolescent offspring rats Authors - Linxi Qian View Article
mouse	WB	Biochim Biophys Acta Mol Basis Dis Inactivation of fatty acid amide hydrolase protects against ischemic reperfusion injury-induced renal fibrogenesis Authors - Chaoling Chen View Article