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FABP4 Polyclonal antibody

FABP4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12802-1-AP

Synonyms

A FABP, AFABP, ALBP, aP2, FABP4, Fatty acid binding protein 4



Tested Applications

Positive WB detected inRAW 264.7 cells, mouse adipose, mouse skeletal muscle, rat heart
Positive IHC detected inhuman breast cancer tissue, rat brown adipose tissue, human ovary tumor tissue, mouse skin tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:4000
Sample-dependent, check data in validation data gallery

Product Information

12802-1-AP targets FABP4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, geese, hamster, pig, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FABP4 fusion protein Ag3912
Full Name fatty acid binding protein 4, adipocyte
Calculated molecular weight 132 aa, 15 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC003672
Gene symbol FABP4
Gene ID (NCBI) 2167
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fatty acid binding protein (FABP) 4 is a member of the FABP family which abundantly expressed, fatty acid carrier proteins. FABPs are capable of binding a variety of hydrophobic molecules such as long-chain fatty acids and are important for their uptake and intracellular trafficking. It was first identified as an adipocyte-specific protein, important for the maintenance of lipid and glucose metabolism. It is also detected in macrophages, where it participates in regulating inflammation and cholesterol trafficking via NFκB and PPAR. In more recent studies, FABP4 has been found in a variety of endothelial cells, where it has been identified as a target of VEGF and a regulator of cell proliferation and possibly angiogenesis. Pathologically, FABP4 has been associated with the development of metabolic syndrome, diabetes and cancer and vulnerability of atherosclerotic plaques. FABP4 has been identified as a novel prognostic factor for both adverse cardiovascular events and breast cancer.

Protocols

Product Specific Protocols
WB protocol for FABP4 antibody 12802-1-APDownload protocol
IHC protocol for FABP4 antibody 12802-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

Tip60-mediated lipin 1 acetylation and ER translocation determine triacylglycerol synthesis rate.

Authors - Terytty Yang Li
mouseWB,IF

Biomaterials

MMP-12 siRNA improves the homeostasis of the small intestine and metabolic dysfunction in high-fat diet feeding-induced obese mice.

Authors - Mingming Song
mouseWB

Cell Rep

Uncoupling of Metabolic Health from Longevity through Genetic Alteration of Adipose Tissue Lipid-Binding Proteins.

Authors - Khanichi N Charles
humanWB,IF

Cell Mol Immunol

PMID: 31363171

Authors - Tian-Yu Lin
humanWB

Stem Cells

miR-223 regulates adipogenic and osteogenic differentiation of mesenchymal stem cells through a C/EBPs/miR-223/FGFR2 regulatory feedback loop.

Authors - Xiaohui Guan
mouseWB

Diabetes

Pygo2 Regulates Adiposity and Glucose Homeostasis via β-Catenin-Axin2-GSK3β Signaling Pathway.

Authors - Yuan-Yuan Xie

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Huai-Chin (Verified Customer) (05-20-2022)

Western blot on 3T3-L1 cells pre and post adipogenesis. Works pretty well. Strong band in 3T3-L1 cells after adipogenesis.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10,000
  • Cell Tissue Type: 3T3-L1