Tested Applications
| Positive WB detected in | RAW 264.7 cells, MC38 cells, RT-4 cells, mouse adipose tissue, mouse skeletal muscle tissue, rat heart tissue, human adipose tissue, rat adipose tissue |
| Positive IHC detected in | mouse skin tissue, human heart tissue, human ovary tumor tissue, rat brown adipose tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12802-1-AP targets FABP4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, bovine, hamster, sheep, goat, geese |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3912 Product name: Recombinant mouse Fabp4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-132 aa of BC054426 Sequence: MCDAFVGTWKLVSSENFDDYMKEVGVGFATRKVAGMAKPNMIISVNGDLVTIRSESTFKNTEISFKLGVEFDEITADDRKVKSIITLDGGALVQVQKWDGKSTTIKRKRDGDKLVVECVMKGVTSTRVYERA Predict reactive species |
| Full Name | fatty acid binding protein 4, adipocyte |
| Calculated Molecular Weight | 132 aa, 15 kDa |
| Observed Molecular Weight | 15 kDa |
| GenBank Accession Number | BC003672 |
| Gene Symbol | FABP4 |
| Gene ID (NCBI) | 2167 |
| RRID | AB_2102442 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P15090 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Fatty acid binding protein (FABP) 4 is a member of the FABP family which abundantly expressed, fatty acid carrier proteins. FABPs are capable of binding a variety of hydrophobic molecules such as long-chain fatty acids and are important for their uptake and intracellular trafficking. It was first identified as an adipocyte-specific protein, important for the maintenance of lipid and glucose metabolism. It is also detected in macrophages, where it participates in regulating inflammation and cholesterol trafficking via NFκB and PPAR. In more recent studies, FABP4 has been found in a variety of endothelial cells, where it has been identified as a target of VEGF and a regulator of cell proliferation and possibly angiogenesis. Pathologically, FABP4 has been associated with the development of metabolic syndrome, diabetes and cancer and vulnerability of atherosclerotic plaques. FABP4 has been identified as a novel prognostic factor for both adverse cardiovascular events and breast cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FABP4 antibody 12802-1-AP | Download protocol |
| IHC protocol for FABP4 antibody 12802-1-AP | Download protocol |
| WB protocol for FABP4 antibody 12802-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-FABP4 antibody (Cat# 12802-1-AP) has accumulated 198 citations across journals including Nature Communications, Cell Metabolism, Cell Death & Differentiation, Advanced Science, Cell Reports, FASEB Journal, Stem Cells, and Metabolism. Research fields span adipogenesis, lipid metabolism, osteogenesis, obesity, cancer biology, atherosclerosis, liver disease, diabetes, stem cell differentiation, and ferroptosis, with applications primarily in Western blot, immunohistochemistry, and immunofluorescence.
| Species | Application | Title |
|---|---|---|
Mol Cancer Targeting OxLDL-mediated CD36 + CAF reprogramming potentiates PD-1 immunotherapy in osteosarcoma | ||
Cell Metab Adipokine IL-11/IL-11Ra constrains sphingolipid metabolism to limit the thermogenic capacity of beige adipocytes. | ||
Bone Res NELL2, a novel osteoinductive factor, regulates osteoblast differentiation and bone homeostasis through fibronectin 1/integrin-mediated FAK/AKT signaling | ||
Autophagy m6A mRNA methylation controls autophagy and adipogenesis by targeting Atg5 and Atg7 | ||
Nat Commun Tip60-mediated lipin 1 acetylation and ER translocation determine triacylglycerol synthesis rate. |
Reviews
What customers say
All five reviews are for Western Blot applications, with an average rating of 4.8/5. Users consistently report clear, specific bands at the expected molecular weight with low background. One reviewer noted a single clean band with no off-target signals. Another highlighted strong signal in 3T3-L1 cells after adipogenesis. Working dilutions ranged from 1:1,000 to 1:10,000. Overall, customers describe it as one of the best-performing antibodies in their lab, praising its high specificity and reliable performance across different cell types and tissues.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Meixin (Verified Customer) (03-10-2026) | This antibody works well for Western blot. I used it to detect the target protein in mouse cell lysates. A clear band was observed at the expected molecular weight with low background signal.
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YINGJIAN (Verified Customer) (11-15-2025) | The antibody produced clear signals with negligible background and high target specificity.
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Mei-xin (Verified Customer) (09-29-2025) | This antibody is very useful. It produces a single band without any other bands. It has a very high specificity.
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Marco (Verified Customer) (07-04-2024) | One of the best working antibodies we have
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Huai-Chin (Verified Customer) (05-20-2022) | Western blot on 3T3-L1 cells pre and post adipogenesis. Works pretty well. Strong band in 3T3-L1 cells after adipogenesis.
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