FADS3 Polyclonal antibody, PBS Only

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  WB analysis using 15205-1-AP (same clone as 15205-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15205-1-AP (FADS3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15205-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 15205-1-AP (same clone as 15205-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15205-1-AP (FADS3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15205-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 15205-1-AP (same clone as 15205-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15205-1-AP (FADS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15205-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 15205-1-AP (same clone as 15205-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15205-1-AP (FADS3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15205-1-PBS in a different storage buffer formulation.

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  IP experiment of L02 using 15205-1-AP (same clone as 15205-1-PBS)

  IP result of anti-FADS3 (IP:15205-1-AP, 4ug; Detection:15205-1-AP 1:500) with L02 cells lysate 3000 ug. This data was developed using the same antibody clone with 15205-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 15205-1-AP (same clone as 15205-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 15205-1-AP (FADS3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15205-1-PBS in a different storage buffer formulation.

FADS3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 15205-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

fatty acid desaturase 3, FADS 3, EC:1.14.19.-, Delta(13) fatty acid desaturase (Delta(13) desaturase ), Cytochrome b5 related protein

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Product Information

15205-1-PBS targets FADS3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FADS3 fusion protein Ag7355
• Full Name: fatty acid desaturase 3
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 41-51 kDa
• GenBank Accession Number: BC004901
• Gene Symbol: FADS3
• Gene ID (NCBI): 3995
• RRID: AB_10859776
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y5Q0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

FADS3, also named as CYB5RP, belongs to fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family memebers are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase protion, both of which are characterized by conserved histidine motifs. FADS3 can be detected as 40 kDa-51 kDa.