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  • KD/KO Validated

FAF1 Polyclonal antibody

FAF1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10271-1-AP

Synonyms

CGI 03, FAF1, FAS associated factor 1, hFAF1, HFAF1s, UBXD12, UBXN3A



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, K-562 cells, mouse brain tissue, mouse testis tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10271-1-AP targets FAF1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAF1 fusion protein Ag0407
Full Name Fas (TNFRSF6) associated factor 1
Calculated molecular weight 74 kDa and 40 kDa
Observed molecular weight 74 kDa
GenBank accession numberBC004970
Gene symbol FAF1
Gene ID (NCBI) 11124
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FAF1 was detected most abundant in testis, slightly less abundant in skeletal muscle and heart, followed by prostate, thymus, ovary, small intestine, and colon, but not in the peripheral blood leukocytes.The N-terminal region (amino acid 1∼201) including the upstream ubiquitin homology domain of hFAF1 could bind with the death domain of Fas, which mediates programmed cell death, also called apoptosis, in a number of organ systems, notably the immune and nervous systems.

Protocols

Product Specific Protocols
WB protocol for FAF1 antibody 10271-1-APDownload protocol
IHC protocol for FAF1 antibody 10271-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Neuroreport

Fas-associated factor 1 promotes in neurofibrillary tangle-mediated cell death of basal forebrain cholinergic neurons in P301L transgenic mice.

Authors - Jong Mu Kim
humanWB

Cancer Manag Res

MiR-26a-5p Serves as an Oncogenic MicroRNA in Non-Small Cell Lung Cancer by Targeting FAF1.

Authors - Ming-Fan Ye
  • KD Validated
mouseWB,IHC,IF

J Neurosci

Fas-associated factor 1 as a regulator of olfactory axon guidance.

Authors - Cheng Kai K
human, mouseWB,IHC,IF

Hum Mol Genet

Accumulation of the parkin substrate, FAF1, plays a key role in the dopaminergic neurodegeneration.

Authors - Sul Jee-Won JW
humanWB,IF

Cell Death Differ

FAF1 mediates regulated necrosis through PARP1 activation upon oxidative stress leading to dopaminergic neurodegeneration.

Authors - Changsun Yu