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FAF2 Polyclonal antibody

FAF2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 16251-1-AP


ETEA, FAF2, FAS associated factor 2, KIAA0887, Protein ETEA, UBXD8, UBXN3B

Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, mouse brain tissue, rat brain tissue, K-562 cells, MCF-7 cells, SH-SY5Y cells, U2OS cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16251-1-AP targets FAF2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAF2 fusion protein Ag9236
Full Name Fas associated factor family member 2
Calculated Molecular Weight 445 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC014001
Gene Symbol FAF2
Gene ID (NCBI) 23197
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein ETEA (FAF2, or UBXD8) is a homolog to Fas-associated factor 1 (FAF1), which is involved in Fas-mediated apoptosis. ETEA protein directly interacts with and negatively regulates neurofibromin. ETEA contains both UBA and UBX domains and overexpression of ETEA downregulates neurofibromin in human cells. It may play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. ETEA is highly expressed in peripheral blood of patients with atopic dermatitis (AD) compared to normal individuals, and may regulate the resistance to apoptosis that is observed in T cells and eosinophils of AD patients.


Product Specific Protocols
WB protocol for FAF2 antibody 16251-1-APDownload protocol
IHC protocol for FAF2 antibody 16251-1-APDownload protocol
IP protocol for FAF2 antibody 16251-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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