FAM83A Polyclonal antibody

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  WB analysis using 29289-1-AP

  DU145 cells were subjected to SDS PAGE followed by western blot with 29289-1-AP (FAM83A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 29289-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29289-1-AP (FAM83A antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 29289-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29289-1-AP (FAM83A antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FAM83A Polyclonal Antibody for WB, IHC, ELISA

Cat No. 29289-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

BJ TSA 9, Protein FAM83A, TSGP, Tumor antigen BJ TSA 9, Tumor antigen BJ-TSA-9

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Tested Applications

• Positive WB detected in: DU 145 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29289-1-AP targets FAM83A in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30946

  Product name: Recombinant human FAM83A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-100 aa of BC007828

  Sequence: MSRSRHLGKIRKRLEDVKSQWVRPARADFSDNESARLATDALLDGGSEAYWRVLSQEGEVDFLSSVEAQYIQAQAREPPCPPDTLGGAEAGPKGLDSSSL

• Full Name: family with sequence similarity 83, member A
• Calculated Molecular Weight: 434 aa, 47 kDa
• Observed Molecular Weight: 47-58 kDa
• GenBank Accession Number: BC007828
• Gene Symbol: FAM83A
• Gene ID (NCBI): 84985
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86UY5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAM83A plays a pivotal role in various biological processes, such as apoptosis, growth, and metastasis. Growing evidence highlights the pro-tumorigenic activity of FAM83A through various signaling pathways, such as the Wnt/β-catenin, ERK, and PI3K/Akt pathways.