Asprosin Polyclonal antibody

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  WB analysis using 20251-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20251-1-AP (Asprosin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary using 20251-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 20251-1-AP (FBN1-Specific Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 20251-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 20251-1-AP (FBN1-Specific Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin cancer using 20251-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 20251-1-AP (FBN1-Specific Antibody) at dilution of 1:50 (under 40x lens).

Asprosin Polyclonal Antibody for WB, IHC, ELISA

Cat No. 20251-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

FBN1-Specific, FBN1, Fibrillin 1 , FBN, Fibrillin-1

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Tested Applications

• Positive WB detected in: A172 cells
• Positive IHC detected in: human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20251-1-AP targets Asprosin in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: fibrillin 1
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: NM_000138
• Gene Symbol: Fibrillin 1
• Gene ID (NCBI): 2200
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35555
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Asprosin is a hormone derived from fibrillin-1 that plays a role in regulating glucose metabolism and appetite. It's released by adipose tissue during fasting and stimulates the liver to release glucose into the bloodstream, ensuring energy availability. Asprosin also influences appetite and body weight by acting on neurons in the hypothalamus. It has been linked to various metabolic diseases, including obesity, type 2 diabetes, and polycystic ovary syndrome (PCOS). The detected molecular size is consistent with that in the article PMID: 35079041.