FBX4 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 87549-1-RR (same clone as 87549-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87549-1-RR (FBX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87549-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 87549-1-RR (same clone as 87549-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using FBX4 antibody (87549-1-RR, Clone: 252858D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87549-1-PBS in a different storage buffer formulation.

FBX4 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 87549-1-PBS

Clone No.252858D2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

FBXO4, F box only protein 4, F box protein 4, F-box only protein 4

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Product Information

87549-1-PBS targets FBX4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag5013

  Product name: Recombinant human FBX4 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-387 aa of BC048098

  Sequence: MAGSEPRSGTNSPPPPFSDWGRLEAAILSGWKTFWQSVSKERVARTTSREEVDEAASTLTRLPIDVQLYILSFLSPHDLCQLGSTNHYWNETVRDPILWRYFLLRDLPSWSSVDWKSLPDLEILKKPISEVTDGAFFDYMAVYRMCCPYTRRASKSSRPMYGAVTSFLHSLIIQNEPRFAMFGPGLEELNTSLVLSLMSSEELCPTAGLPQRQIDGIGSGVNFQLNNQHKFNILILYSTTRKERDRAREEHTSAVNKMFSRHNEGDDQQGSRYSVIPQIQKVCEVVDGFIYVANAEAHKRHEWQDEFSHIMAMTDPAFGSSGRPLLVLSCISQGDVKRMPCFYLAHELHLNLLNHPWLVQDTEAETLTGFLNGIEWILEEVESKRAR

• Full Name: F-box protein 4
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC048098
• Gene Symbol: FBXO4/FBX4
• Gene ID (NCBI): 26272
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UKT5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

FBX4 (F-Box Protein 4), also known as FBXO4, is a member of the F-box protein family. FBX4 is involved in the ubiquitination of various target proteins, leading to their degradation. It has been shown to regulate the cell cycle by targeting proteins such as cyclin D1 for ubiquitination and degradation. Mutations in FBX4 have been linked to various diseases, including spermatogenic failure and esophageal cancer. In cancer, FBX4 has been shown to act as a tumor suppressor. Loss of FBX4 function can lead to increased cell proliferation and tumor development. For example, FBX4 knockout mice have been shown to develop various tumors, including lymphoblastic lymphoma and mammary carcinoma. FBX4 has two isforms: 44 kDa and 35 kDa. The observd molecular weight is consistent with the result in PMID: 21242966.