FBX4 Polyclonal antibody

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  IP experiment of HeLa using 13855-1-AP

  IP result of anti-FBX4 (IP:13855-1-AP, 4ug; Detection:13855-1-AP 1:800) with HeLa cells lysate 1400 ug.

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  IP experiment of A431 using 13855-1-AP

  IP result of anti-FBX4 (IP:13855-1-AP, 4ug; Detection:13855-1-AP 1:800) with A431 cells lysate 1040 ug.

FBX4 Polyclonal Antibody for IP, ELISA

Cat No. 13855-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IP, ELISA

Synonyms

FBXO4, F-box only protein 4, F box protein 4, F box only protein 4

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Tested Applications

• Positive IP detected in: HeLa cells, A431 cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13855-1-AP targets FBX4 in IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FBX4 fusion protein Ag4829
• Full Name: F-box protein 4
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC048098
• Gene Symbol: FBXO4/FBX4
• Gene ID (NCBI): 26272
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UKT5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBX4 (F-Box Protein 4), also known as FBXO4, is a member of the F-box protein family. FBX4 is involved in the ubiquitination of various target proteins, leading to their degradation. It has been shown to regulate the cell cycle by targeting proteins such as cyclin D1 for ubiquitination and degradation. Mutations in FBX4 have been linked to various diseases, including spermatogenic failure and esophageal cancer. In cancer, FBX4 has been shown to act as a tumor suppressor. Loss of FBX4 function can lead to increased cell proliferation and tumor development. For example, FBX4 knockout mice have been shown to develop various tumors, including lymphoblastic lymphoma and mammary carcinoma.