FGF9 Polyclonal antibody

FGF9 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26554-1-AP

Synonyms

FGF-9, FGF 9, Heparin-binding growth factor 9, HBGF-9, Glia-activating factor

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Validation Data Gallery

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  IHC staining of human stomach using 26554-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 26554-1-AP (FGF9 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach using 26554-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 26554-1-AP (FGF9 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human small intestine using 26554-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 26554-1-AP (FGF9 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 26554-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 26554-1-AP (FGF9 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 26554-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26554-1-AP (FGF9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 26554-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26554-1-AP (FGF9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 26554-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26554-1-AP (FGF9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 26554-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26554-1-AP (FGF9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 26554-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using FGF9 antibody (26554-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive IHC detected in: human stomach tissue, human prostate cancer tissue, human colon cancer tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26554-1-AP targets FGF9 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FGF9 fusion protein Ag24244
• Full Name: fibroblast growth factor 9 (glia-activating factor)
• Calculated Molecular Weight: 208 aa, 23 kDa
• GenBank Accession Number: BC069692
• Gene Symbol: FGF9
• Gene ID (NCBI): 2254
• RRID: AB_2880550
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibroblast growth factor 9 (FGF9) is a critically important and elegantly regulated growth factor that was first identified during a screen for factors acting on cells of the central nervous system (CNS). Over the subsequent two decades of research, FGF9 has been revealed to play a significant role in various biological processes. A key feature of its control is reciprocal compartmentalization, particularly during development, with the epithelium serving as a dominant source and mesenchyme as a prime target. This mesenchyme selectivity is achieved through the high affinity of FGF9 to the IIIc isoforms of FGFR1, 2, and 3. FGF9 is widely expressed in the embryo, including in the developing heart and lungs, and more selectively in adult tissues, such as the CNS and kidneys.