FGFR4 Polyclonal antibody

FGFR4 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11098-1-AP

Synonyms

CD334, FGFR 4, FGFR4, JTK2, TKF



Tested Applications

Positive WB detected inHepG2 cells, mouse lung tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11098-1-AP targets FGFR4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FGFR4 fusion protein Ag1573
Full Name fibroblast growth factor receptor 4
Calculated molecular weight 88 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC011847
Gene symbol FGFR4
Gene ID (NCBI) 2264
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FGFR4 antibody 11098-1-APDownload protocol
IHC protocol for FGFR4 antibody 11098-1-APDownload protocol
IF protocol for FGFR4 antibody 11098-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Hepatol Commun

FGF1 Signaling Modulates Biliary Injury and Liver Fibrosis in the Mdr2-/- Mouse Model of Primary Sclerosing Cholangitis.

Authors - April O'Brien
humanIHC

Cancer Res Treat

FGFR4 Gly388Arg Polymorphism Affects the Progression of Gastric Cancer by Activating STAT3 Pathway to Induce Epithelial to Mesenchymal Transition.

Authors - Yanwei Ye
humanIHC, WB

Int J Oncol

Role of fibroblast growth factor 4 in the growth and metastasis of colorectal cancer.

Authors - Yanwei Ye
human

J Cell Biochem

Glycosylation of FGFR4 in cholangiocarcinoma regulates receptor processing and cancer signaling.

Authors - Andrew J Phillips
mouseWB

Biomed Pharmacother

Bupleuri radix extract ameliorates impaired lipid metabolism in high-fat diet-induced obese mice via gut microbia-mediated regulation of FGF21 signaling pathway.

Authors - Liucen Wu
mouseWB

Biochem Pharmacol

Association between the perturbation of bile acid homeostasis and valproic acid-induced hepatotoxicity.

Authors - Yanan Chen