FIS1 Monoclonal antibody
FIS1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human and More (4)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
CloneNo.
2F10E1
Cat no : 66635-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | LNCaP cells, HEK-293 cells, HeLa cells, MCF-7 cells, Jurkat cells, HepG2 cells, K-562 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human breast cancer tissue, human prostate cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 11 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
Product Information
66635-1-Ig targets FIS1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat, goat, fish |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | FIS1 fusion protein Ag1409 |
Full Name | fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae) |
Calculated Molecular Weight | 17 kDa |
Observed Molecular Weight | 15 kDa |
GenBank Accession Number | BC009428 |
Gene Symbol | FIS1 |
Gene ID (NCBI) | 51024 |
RRID | AB_2881994 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Fis1 (fission 1) is an integral mitochondrial outer membrane protein that participates in mitochondrial fission by interacting with dynamin-related protein 1 (Drp1). Excessive mitochondrial fission is associated with the pathology of a number of neurodegenerative or neurodevelopmental diseases. Increased expression of Fis1 has been found in Huntington's disease (HD)-affected brain, Alzheimer's disease (AD) patients, and autism spectrum disorder. (21257639, 21459773, 23333625)
Protocols
Product Specific Protocols | |
---|---|
WB protocol for FIS1 antibody 66635-1-Ig | Download protocol |
IHC protocol for FIS1 antibody 66635-1-Ig | Download protocol |
IF protocol for FIS1 antibody 66635-1-Ig | Download protocol |
IP protocol for FIS1 antibody 66635-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Pineal Res Melatonin and verteporfin synergistically suppress the growth and stemness of head and neck squamous cell carcinoma through the regulation of mitochondrial dynamics | ||
Front Immunol Metformin Modulates T Cell Function and Alleviates Liver Injury Through Bioenergetic Regulation in Viral Hepatitis. | ||
J Physiol Sci Cardioprotective responses to aerobic exercise-induced physiological hypertrophy in zebrafish heart | ||
Biol Pharm Bull Ampelopsin inhibits breast cancer cell growth through mitochondrial apoptosis pathway | ||
J Mol Histol Roles of HIF-1α/BNIP3 mediated mitophagy in mitochondrial dysfunction of letrozole-induced PCOS rats | ||
Aging (Albany NY) Jian-Pi-Yi-Shen decoction inhibits mitochondria-dependent granulosa cell apoptosis in a rat model of POF |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Chun (Verified Customer) (06-19-2022) | This antibody worked very well.
|