FIS1 Monoclonal antibody

FIS1 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

2F10E1

Cat no : 66635-1-Ig

Synonyms

CGI 135, FIS1, FIS1 homolog, hFis1, TPR repeat protein 11, TTC11



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, MCF-7 cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

66635-1-Ig targets FIS1 in WB, IHC, IF,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FIS1 fusion protein Ag1409
Full Name fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae)
Calculated molecular weight 17 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC009428
Gene symbol FIS1
Gene ID (NCBI) 51024
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fis1 (fission 1) is an integral mitochondrial outer membrane protein that participates in mitochondrial fission by interacting with dynamin-related protein 1 (Drp1). Excessive mitochondrial fission is associated with the pathology of a number of neurodegenerative or neurodevelopmental diseases. Increased expression of Fis1 has been found in Huntington’s disease (HD)-affected brain, Alzheimer’s disease (AD) patients, and autism spectrum disorder. (21257639, 21459773, 23333625)

Protocols

Product Specific Protocols
WB protocol for FIS1 antibody 66635-1-IgDownload protocol
IHC protocol for FIS1 antibody 66635-1-IgDownload protocol
IF protocol for FIS1 antibody 66635-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Immunol

Metformin Modulates T Cell Function and Alleviates Liver Injury Through Bioenergetic Regulation in Viral Hepatitis.

Authors - Lanman Xu