FIS1 Monoclonal antibody

FIS1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (5)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2F10E1

Cat no : 66635-1-Ig

Synonyms

CGI 135, FIS1, FIS1 homolog, hFis1, TPR repeat protein 11, TTC11



Tested Applications

Positive WB detected inHEK-293 cells, LNCaP cells, HeLa cells, MCF-7 cells, Jurkat cells, HepG2 cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66635-1-Ig targets FIS1 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse, rat, goat, fish
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FIS1 fusion protein Ag1409
Full Name fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae)
Calculated Molecular Weight 17 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC009428
Gene Symbol FIS1
Gene ID (NCBI) 51024
RRIDAB_2881994
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fis1 (fission 1) is an integral mitochondrial outer membrane protein that participates in mitochondrial fission by interacting with dynamin-related protein 1 (Drp1). Excessive mitochondrial fission is associated with the pathology of a number of neurodegenerative or neurodevelopmental diseases. Increased expression of Fis1 has been found in Huntington's disease (HD)-affected brain, Alzheimer's disease (AD) patients, and autism spectrum disorder. (21257639, 21459773, 23333625)

Protocols

Product Specific Protocols
WB protocol for FIS1 antibody 66635-1-IgDownload protocol
IHC protocol for FIS1 antibody 66635-1-IgDownload protocol
IF protocol for FIS1 antibody 66635-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Pineal Res

Melatonin and verteporfin synergistically suppress the growth and stemness of head and neck squamous cell carcinoma through the regulation of mitochondrial dynamics

Authors - Ye Young Shin
mouseWB

Front Immunol

Metformin Modulates T Cell Function and Alleviates Liver Injury Through Bioenergetic Regulation in Viral Hepatitis.

Authors - Lanman Xu
fishWB

J Physiol Sci

Cardioprotective responses to aerobic exercise-induced physiological hypertrophy in zebrafish heart

Authors - Zhanglin Chen
humanIF

Biol Pharm Bull

Ampelopsin inhibits breast cancer cell growth through mitochondrial apoptosis pathway

Authors - YUE LI
ratIHC

J Mol Histol

Roles of HIF-1α/BNIP3 mediated mitophagy in mitochondrial dysfunction of letrozole-induced PCOS rats

Authors - Fan Wang
ratWB

Aging (Albany NY)

Jian-Pi-Yi-Shen decoction inhibits mitochondria-dependent granulosa cell apoptosis in a rat model of POF

Authors - Xiao-Lin Jiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Chun (Verified Customer) (06-19-2022)

This antibody worked very well.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1,000
  • Cell Tissue Type: HeLa cells and HEK293 cells