FKBP10/FKBP65 Polyclonal antibody

• Featured Product
• KD/KO Validated

FKBP10/FKBP65 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

20

Reviews (1)

Cat no : 12172-1-AP

Synonyms

FKBP10, FKBP65, FKBP 65, FKBP 10, FK506-binding protein 10

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Validation Data Gallery

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  WB analysis using 12172-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12172-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12172-1-AP

  WB result of FKBP10/FKBP65 antibody (12172-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FKBP10/FKBP65 transfected HeLa cells.

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  WB analysis of human brain using 12172-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 12172-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12172-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 12172-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 12172-1-AP

  IP result of anti-FKBP10/FKBP65 (IP:12172-1-AP, 4ug; Detection:12172-1-AP 1:600) with HEK-293 cells lysate 2800ug.

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  IHC staining of human ovary cancer using 12172-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 12172-1-AP (FKBP10/FKBP65 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 12172-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 12172-1-AP (FKBP10/FKBP65 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, human brain tissue, human placenta tissue, human kidney tissue, fetal human brain tissue, HeLa cells, NIH/3T3 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 18 publications below
• IHC: See 2 publications below
• IF: See 6 publications below

Product Information

12172-1-AP targets FKBP10/FKBP65 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FKBP10/FKBP65 fusion protein Ag2814
• Full Name: FK506 binding protein 10, 65 kDa
• Calculated Molecular Weight: 582 aa, 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC016467
• Gene Symbol: FKBP10
• Gene ID (NCBI): 60681
• RRID: AB_2102550
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FKBP10, also known as FKBP65, is a 65 kDa FK506 binding protein that is a member of the FKBP-type peptidyl-prolyl cis/trans isomerase family. This protein localizes to the endoplasmic reticulum and acts as a molecular chaperone and binding partner of type I collagen. FKBP65 is expressed in developing tissues and re-expressed in adult tissues following injury. As documented, FKBP10 has been detected to play important roles in the initiation and progression of cancers.

Publications

Species	Application	Title
mouse	WB	Mol Cell; Proteomic mapping of the human mitochondrial intermembrane space in live cells via ratiometric APEX tagging.; Authors - Victoria Hung
human,mouse	WB	Sci Adv; Melanoma-intrinsic NR2F6 activity regulates antitumor immunity; Authors - Hyungsoo Kim ; KD Validated
human	WB,IF	Proc Natl Acad Sci U S A; Disentangling mechanisms involved in collagen pyridinoline cross-linking: The immunophilin FKBP65 is critical for dimerization of lysyl hydroxylase 2.; Authors - Rutger A F Gjaltema ; KD Validated
mouse	IHC	Proc Natl Acad Sci U S A; Localized chondro-ossification underlies joint dysfunction and motor deficits in the Fkbp10 mouse model of osteogenesis imperfecta.; Authors - Joohyun Lim
mouse	WB,IF	Hum Mol Genet; Connective Tissue Alterations in Fkbp10-/- mice.; Authors - Caressa D Lietman
human	WB, IF	Hum Mol Genet; HSP47 and FKBP65 cooperate in the synthesis of type I procollagen.; Authors - Ivan Duran

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Doug (Verified Customer) (10-08-2019)

Excellent antibody for western blot.Validated by shFKBP10.

• Applications: Western Blot,
• Primary Antibody Dilution: 1000X
• Cell Tissue Type: Cell line

FKBP10/FKBP65 Antibody Western Blot, validation (1000X dilution) in Cell line (Cat no:12172-1-AP)