Tested Applications
| Positive WB detected in | HEK-293 cells, human brain tissue, human placenta tissue, human kidney tissue, fetal human brain tissue, HeLa cells, NIH/3T3 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12172-1-AP targets FKBP10/FKBP65 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2814 Product name: Recombinant human FKBP10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 233-582 aa of BC016467 Sequence: PFLAYGEKGYGTVIPPQASLVFHVLLIDVHNPKDAVQLETLELPPGCVRRAGAGDFMRYHYNGSLMDGTLFDSSYSRNHTYNTYIGQGYIIPGMDQGLQGACMGERRRITIPPHLAYGENGTGDKIPGSAVLIFNVHVIDFHNPADVVEIRTLSRPSETCNETTKLGDFVRYHYNCSLLDGTQLFTSHDYGAPQEATLGANKVIEGLDTGLQGMCVGERRQLIVPPHLAHGESGARGVPGSAVLLFEVELVSREDGLPTGYLFVWHKDPPANLFEDMDLNKDGEVPPEEFSTFIKAQVSEGKGRLMPGQDPEKTIGDMFQNQDRNQDGKITVDELKLKSDEDEERVHEEL Predict reactive species |
| Full Name | FK506 binding protein 10, 65 kDa |
| Calculated Molecular Weight | 582 aa, 64 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC016467 |
| Gene Symbol | FKBP10 |
| Gene ID (NCBI) | 60681 |
| RRID | AB_2102550 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96AY3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FKBP10, also known as FKBP65, is a 65 kDa FK506 binding protein that is a member of the FKBP-type peptidyl-prolyl cis/trans isomerase family. This protein localizes to the endoplasmic reticulum and acts as a molecular chaperone and binding partner of type I collagen. FKBP65 is expressed in developing tissues and re-expressed in adult tissues following injury. As documented, FKBP10 has been detected to play important roles in the initiation and progression of cancers.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for FKBP10/FKBP65 antibody 12172-1-AP | Download protocol |
| IP protocol for FKBP10/FKBP65 antibody 12172-1-AP | Download protocol |
| WB protocol for FKBP10/FKBP65 antibody 12172-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FKBP10 antibody (Cat# 12172-1-AP) has 29 citations published in journals including Molecular Cell, PNAS, Cell Reports, Science Advances, Journal of Bone and Mineral Research, Human Molecular Genetics, and Scientific Reports. Research fields span collagen biosynthesis and post-translational modifications, osteogenesis imperfecta, fibrosis (pulmonary, hepatic, muscular), cancer biology (melanoma, bladder, lung, gastric, hepatocellular carcinoma), endoplasmic reticulum stress, and tumor immunosuppression. Applications include WB, IHC, IF, IP, and CoIP.
| Species | Application | Title |
|---|---|---|
Mol Cell Proteomic mapping of the human mitochondrial intermembrane space in live cells via ratiometric APEX tagging. | ||
Acta Pharm Sin B RDYH58 functional exosomes targeting myofibroblasts loaded with siFKBP10 for inhibition of collagen biosynthesis and secretion of IPF. | ||
Cell Rep Med Personalized drug screening using patient-derived organoid and its clinical relevance in gastric cancer | ||
Int J Biol Sci FKBP10 Promotes the Muscle Invasion of Bladder Cancer via Lamin A Dysregulation | ||
Proc Natl Acad Sci U S A Disentangling mechanisms involved in collagen pyridinoline cross-linking: The immunophilin FKBP65 is critical for dimerization of lysyl hydroxylase 2.
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Reviews
What customers say
The single review for this product is a 5-star rating from a Western Blot application using a 1:1000 dilution on a cell line. The reviewer described it as an excellent antibody for Western blot and noted that it was validated by shFKBP10 knockdown, confirming its specificity and reliability for detecting the target protein.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Doug (Verified Customer) (10-08-2019) | Excellent antibody for western blot.Validated by shFKBP10.
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