FKBP52 Monoclonal antibody, PBS Only (Capture)

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  WB analysis using 66040-2-Ig (same clone as 66040-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66040-2-Ig (FKBP52 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66040-2-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66040-2-Ig (same clone as 66040-2-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66040-2-Ig (FKBP52 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66040-2-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66040-2-Ig (same clone as 66040-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66040-2-Ig (FKBP52 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66040-2-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66040-2-Ig (same clone as 66040-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66040-2-Ig (FKBP52 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66040-2-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 66040-2-Ig (same clone as 66040-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using FKBP52 antibody (66040-2-Ig, Clone: 1B5F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66040-2-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 66040-2-Ig (same clone as 66040-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using FKBP52 antibody (66040-2-Ig, Clone: 1B5F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66040-2-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP51486-1

  Cytometric bead array sample test of MP51486-1, FKBP4 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66040-2-PBS. Detection antibody: 66040-3-PBS.

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  Cytometric bead array standard curve of MP51486-1

  Cytometric bead array standard curve of MP51486-1, FKBP4 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66040-2-PBS. Detection antibody: 66040-3-PBS. Standard:Ag18704. Range: 0.781-100 ng/mL.

FKBP52 Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Cat No. 66040-2-PBS

Clone No.1B5F1

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Synonyms

FKBP4, 1B5F1, 51 kDa FK506-binding protein, 52 kDa FK506-binding protein, EC:5.2.1.8

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Product Information

66040-2-PBS targets FKBP52 as part of a matched antibody pair:

MP51486-1: 66040-2-PBS capture and 66040-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18704

  Product name: Recombinant human FKBP52 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-286 aa of BC007924

  Sequence: MTAEEMKATESGAQSAPLPMEGVDISPKQDEGVLKVIKREGTGTEMPMIGDRVFVHYTGWLLDGTKFDSSLDRKDKFSFDLGKGEVIKAWDIAIATMKVGEVCHITCKPEYAYGSAGSPPKIPPNATLVFEVELFEFKGEDLTEEEDGGIIRRIQTRGEGYAKPNEGAIVEVALEGYYKDKLFDQRELRFEIGEGENLDLPYGLERAIQRMEKGEHSIVYLKPSYAFGSVGKEKFQIPPNAELKYELHLKSFEKAKESWEMNSEEKLEQSTIVKERGTVYFKEGKY

• Full Name: FK506 binding protein 4, 59kDa
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC007924
• Gene Symbol: FKBP52
• Gene ID (NCBI): 2288
• RRID: AB_11043344
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q02790
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

FKBP52 (FK506 binding protein 4) is a member of the immunophilin protein family that comprises intracellular protein effectors of immunosuppressive drugs, and it is known as a steroid receptor-associated protein. As a novel regulator of microtubule dynamics, FKBP52 is associated with the motor protein dynein and with the cytoskeleton during mitosis. FKBP52 is highly expressed in CNS regions susceptible to Alzheimer's, and plays a role in modulating toxicity of Abeta peptides. The protein can associate with the Tau function, and may help to decipher and modulate the events involved in Tau-induced neurodegeneration. In addition, FKBP52 is likely to play a role in growth and development of the male genitalia.