FLT3 Polyclonal antibody

FLT3 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 21049-1-AP

Synonyms

CD135, FL cytokine receptor, FLK2, FLT 3, FLT3, Fms like tyrosine kinase 3, fms related tyrosine kinase 3, Stem cell tyrosine kinase 1, STK 1, STK1



Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21049-1-AP is FLT3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FLT3 fusion protein Ag15391
Full Name fms-related tyrosine kinase 3
Calculated molecular weight 993 aa, 113 kDa
Observed molecular weight 130-140 kDa
GenBank accession numberBC036028
Gene symbol FLT3
Gene ID (NCBI) 2322
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FLT3 (also known as CD135 or FLK2) is a tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. FLT3 was originally identified by its expression in hematopoietic stem/progenitor cells (PMID: 7507245). It is important for the normal development of hematopoietic stem/progenitor cells. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.

Protocols

Product Specific Protocols
WB protocol for FLT3 antibody 21049-1-APDownload protocol
IP protocol for FLT3 antibody 21049-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Med Mol Morphol

An analysis of monocytes and dendritic cells differentiated from human peripheral blood monocyte-derived induced pluripotent stem cells.

Authors - Noriko Hiramatsu
mouseWB,IF

Biochem Biophys Res Commun

The angiogenic factor Egfl7 alters thymogenesis by activating Flt3 signaling.

Authors - Yousef Salama