FLT3 Polyclonal antibody

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  WB analysis using 21049-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21049-1-AP (FLT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21049-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21049-1-AP (FLT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 21049-1-AP

  IP result of anti-FLT3 (IP:21049-1-AP, 4ug; Detection:21049-1-AP 1:500) with mouse brain tissue lysate 2640ug.

FLT3 Polyclonal Antibody for WB, IP, ELISA

Cat No. 21049-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IP, ELISA

Synonyms

CD135, FL cytokine receptor, FLK2, FLT 3, FLT3, Fms like tyrosine kinase 3, fms related tyrosine kinase 3, Stem cell tyrosine kinase 1, STK 1, STK1

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

21049-1-AP targets FLT3 in WB, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15391

  Product name: Recombinant human FLT3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 27-376 aa of BC036028

  Sequence: NQDLPVIKCVLINHKNNDSSVGKSSSYPMVSESPEDLGCALRPQSSGTVYEAAAVEVDVSASITLQVLVDAPGNISCLWVFKHSSLNCQPHFDLQNRGVVSMVILKMTETQAGEYLLFIQSEATNYTILFTVSIRNTLLYTLRRPYFRKMENQDALVCISESVPEPIVEWVLCDSQGESCKEESPAVVKKEEKVLHELFGTDIRCCARNELGRECTRLFTIDLNQTPQTTLPQLFLKVGEPLWIRCKAVHVNHGFGLTWELENKALEEGNYFEMSTYSTNRTMIRILFAFVSSVARNDTGYYTCSSSKHPSQSALVTIVEKGFINATNSSEDYEIDQYEEFCFSVRFKAY

• Full Name: fms-related tyrosine kinase 3
• Calculated Molecular Weight: 993 aa, 113 kDa
• Observed Molecular Weight: 140-160 kDa
• GenBank Accession Number: BC036028
• Gene Symbol: FLT3
• Gene ID (NCBI): 2322
• RRID: AB_10733759
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P36888
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FLT3 (also known as CD135 or FLK2) is a tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation, and survival of hematopoietic progenitor cells and of dendritic cells. FLT3 was originally identified by its expression in hematopoietic stem/progenitor cells (PMID: 7507245). It is important for the normal development of hematopoietic stem/progenitor cells. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Cell Rep; Activation of GPR44 decreases severity of myeloid leukemia via specific targeting of leukemia initiating stem cells; Authors - Fenghua Qian
human	WB,IP	J Med Chem; Proteolysis-Targeting Chimera (PROTAC) Modification of Dovitinib Enhances the Antiproliferative Effect against FLT3-ITD-Positive Acute Myeloid Leukemia Cells; Authors - Sheng Cao
human	WB	Bioorg Med Chem; Synthesis and biological evaluation of 4-(4-aminophenyl)-6-methylisoxazolo[3,4-b] pyridin-3-amine covalent inhibitors as potential agents for the treatment of acute myeloid leukemia.; Authors - Ji-Bo Kang
mouse	WB,IF	Biochem Biophys Res Commun; The angiogenic factor Egfl7 alters thymogenesis by activating Flt3 signaling.; Authors - Yousef Salama
human		Med Mol Morphol; An analysis of monocytes and dendritic cells differentiated from human peripheral blood monocyte-derived induced pluripotent stem cells.; Authors - Noriko Hiramatsu
mouse	WB	FASEB J; Activation of FMS-like tyrosine kinase 3 protects against isoprenaline-induced cardiac hypertrophy by improving autophagy and mitochondrial dynamics; Authors - Xixi Jiang