FLT3 Polyclonal antibody

FLT3 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 21049-1-AP


CD135, FL cytokine receptor, FLK2, FLT 3, FLT3, Fms like tyrosine kinase 3, fms related tyrosine kinase 3, Stem cell tyrosine kinase 1, STK 1, STK1

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21049-1-AP targets FLT3 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FLT3 fusion protein Ag15391
Full Name fms-related tyrosine kinase 3
Calculated Molecular Weight 993 aa, 113 kDa
Observed Molecular Weight140-160 kDa
GenBank Accession NumberBC036028
Gene Symbol FLT3
Gene ID (NCBI) 2322
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FLT3 (also known as CD135 or FLK2) is a tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation, and survival of hematopoietic progenitor cells and of dendritic cells. FLT3 was originally identified by its expression in hematopoietic stem/progenitor cells (PMID: 7507245). It is important for the normal development of hematopoietic stem/progenitor cells. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.


Product Specific Protocols
WB protocol for FLT3 antibody 21049-1-APDownload protocol
IP protocol for FLT3 antibody 21049-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Rep

Activation of GPR44 decreases severity of myeloid leukemia via specific targeting of leukemia initiating stem cells

Authors - Fenghua Qian

J Med Chem

Proteolysis-Targeting Chimera (PROTAC) Modification of Dovitinib Enhances the Antiproliferative Effect against FLT3-ITD-Positive Acute Myeloid Leukemia Cells

Authors - Sheng Cao

Bioorg Med Chem

Synthesis and biological evaluation of 4-(4-aminophenyl)-6-methylisoxazolo[3,4-b] pyridin-3-amine covalent inhibitors as potential agents for the treatment of acute myeloid leukemia.

Authors - Ji-Bo Kang

Biochem Biophys Res Commun

The angiogenic factor Egfl7 alters thymogenesis by activating Flt3 signaling.

Authors - Yousef Salama

Med Mol Morphol

An analysis of monocytes and dendritic cells differentiated from human peripheral blood monocyte-derived induced pluripotent stem cells.

Authors - Noriko Hiramatsu


Activation of FMS-like tyrosine kinase 3 protects against isoprenaline-induced cardiac hypertrophy by improving autophagy and mitochondrial dynamics

Authors - Xixi Jiang