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Fibronectin Monoclonal antibody
Fibronectin Monoclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC, CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
1G10F9
Cat no : 66042-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human plasma tissue, rat serum tissue, human brain tissue, human liver tissue |
| Positive IP detected in | human plasma tissue |
| Positive IHC detected in | human colon cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 25 publications below |
| IHC | See 10 publications below |
| IF | See 6 publications below |
| FC | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
The immunogen of 66042-1-Ig is Fibronectin Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Fibronectin fusion protein Ag8016 |
| Full Name | fibronectin 1 |
| Calculated molecular weight | 2386 aa, 263 kDa |
| Observed molecular weight | 263 kDa |
| GenBank accession number | BC005858 |
| Gene symbol | FN1 |
| Gene ID (NCBI) | 2335 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Fibronectin 1 (FN1) is a high molecular weight glycoprotein which exists in both a soluble form in plasma (plasma FN1) and other body fluids and an insoluble form in the extracellular matrix (cellular FN1). Plasma FN1 (dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. FN1 binds to cell surfaces through integrins and to various compounds including collagen, fibrin and heparin. It is involved in cell adhesion and migration processes including embryogenesis, wound healing, hemostasis, host defense, and metastasis. (PMID: 7276612; 12244123; 12847088)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Fibronectin antibody 66042-1-Ig | Download protocol |
| IHC protocol for Fibronectin antibody 66042-1-Ig | Download protocol |
| IF protocol for Fibronectin antibody 66042-1-Ig | Download protocol |
| IP protocol for Fibronectin antibody 66042-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cytotechnology Angiopoietin-like protein 1 inhibits epithelial to mesenchymal transition in colorectal cancer cells via suppress Slug expression. | ||
Int J Mol Sci Differential Angiogenic Potential of 3-Dimension Spheroid of HNSCC Cells in Mouse Xenograft. | ||
Cell Cycle Quercetin prevents isoprenaline-induced myocardial fibrosis by promoting autophagy via regulating miR-223-3p/FOXO3. | ||
Cell Death Discov ORP5 promotes tumor metastasis via stabilizing c-Met in renal cell carcinoma. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Deqiang (Verified Customer) (05-14-2020) | it works well on mouse embryo paraffin sections.
|
FH Jihyun (Verified Customer) (05-07-2020) | There is no background signal. I can get a good one band in the WB. Great!
|
