- Featured Product
- KD/KO Validated
Fibronectin Monoclonal antibody
Fibronectin Monoclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 66042-1-Ig
Validation Data Gallery
|Positive WB detected in
|human plasma tissue, human plasma, rat serum, HuH-7 cells, HepG2 cells, MG-63 cells, U2OS cells
|Positive IP detected in
|human plasma tissue
|Positive IHC detected in
|human colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|NIH/3T3 cells, human colon cancer tissue
|Western Blot (WB)
|WB : 1:2000-1:12000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:300-1:1200
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66042-1-Ig targets Fibronectin in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat
|Host / Isotype
|Mouse / IgG1
|Fibronectin fusion protein Ag8016
|Calculated molecular weight
|2386 aa, 263 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Fibronectin 1 (FN1) is a high molecular weight glycoprotein which exists in both a soluble form in plasma (plasma FN1) and other body fluids and an insoluble form in the extracellular matrix (cellular FN1). Plasma FN1 (dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. FN1 binds to cell surfaces through integrins and to various compounds including collagen, fibrin and heparin. It is involved in cell adhesion and migration processes including embryogenesis, wound healing, hemostasis, host defense, and metastasis. (PMID: 7276612; 12244123; 12847088)
|Product Specific Protocols
|WB protocol for Fibronectin antibody 66042-1-Ig
|IHC protocol for Fibronectin antibody 66042-1-Ig
|IF protocol for Fibronectin antibody 66042-1-Ig
|IP protocol for Fibronectin antibody 66042-1-Ig
|Click here to view our Standard Protocols
Int J Biol Macromol
A multifunctional polydopamine/genipin/alendronate nanoparticle licences fibrin hydrogels osteoinductive and immunomodulatory potencies for repairing bone defects
Nintedanib prevents TGF-β2-induced epithelial-mesenchymal transition in retinal pigment epithelial cells
Oxid Med Cell Longev
HBO1 as an Important Target for the Treatment of CCL4-Induced Liver Fibrosis and Aged-Related Liver Aging and Fibrosis
Ecotoxicol Environ Saf
Alleviative effect of melatonin against the nephrotoxicity induced by cadmium exposure through regulating renal oxidative stress, inflammatory reaction, and fibrosis in a mouse model
Accumulation of polystyrene microplastics induces liver fibrosis by activating cGAS/STING pathway.
Int J Mol Sci
Differential Angiogenic Potential of 3-Dimension Spheroid of HNSCC Cells in Mouse Xenograft.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Udesh (Verified Customer) (04-19-2023)
Worked well for IP, band seen just below 250 kD (page ruler plus ladder). Lower band is light chain IgG
Ren Jie (Verified Customer) (07-11-2022)
Quite significant smear and non-specific binding
Deqiang (Verified Customer) (05-14-2020)
it works well on mouse embryo paraffin sections.
Jihyun (Verified Customer) (05-07-2020)
There is no background signal. I can get a good one band in the WB. Great!