Fibronectin Monoclonal antibody

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  WB analysis of human plasma using 66042-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66042-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HuH-7 using 66042-1-Ig

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 66042-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of MG-63 using 66042-1-Ig

  MG-63 cells were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat serum using 66042-1-Ig

  rat serum were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of human plasma using 66042-1-Ig

  IP result of anti-Fibronectin (IP:66042-1-Ig, 5ug; Detection:66042-1-Ig 1:500) with human plasma lysate 4000ug.

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  IHC staining of human colon cancer using 66042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human colon cancer using 66042-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Fibronectin antibody (66042-1-Ig, Clone: 1G10F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human colon cancer using 66042-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Fibronectin antibody (66042-1-Ig, Clone: 1G10F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of NIH/3T3 using 66042-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Fibronectin antibody (66042-1-Ig, Clone: 1G10F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of NIH/3T3 using 66042-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol ) fixed NIH/3T3 cells using 66042-1-Ig(Fibronectin antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• Featured Product
• KD/KO Validated

Fibronectin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 66042-1-Ig

Clone No.1G10F9

Publications(140)

Reviews (4)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA, Cell treatment

Synonyms

FN1, 1G10F9, Anastellin, CIG, Cold insoluble globulin

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Tested Applications

• Positive WB detected in: human plasma tissue, human plasma, rat serum, HuH-7 cells, HepG2 cells, MG-63 cells, U2OS cells
• Positive IP detected in: human plasma tissue
• Positive IHC detected in: human colon cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human colon cancer tissue
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 91 publications below
• IHC: See 39 publications below
• IF: See 40 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Product Information

66042-1-Ig targets Fibronectin in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, sheep
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Fibronectin fusion protein Ag8016
• Full Name: fibronectin 1
• Calculated Molecular Weight: 2386 aa, 263 kDa
• Observed Molecular Weight: 263 kDa
• GenBank Accession Number: BC005858
• Gene Symbol: Fibronectin
• Gene ID (NCBI): 2335
• RRID: AB_11182385
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P02751
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibronectin 1 (FN1) is a high molecular weight glycoprotein which exists in both a soluble form in plasma (plasma FN1) and other body fluids and an insoluble form in the extracellular matrix (cellular FN1). Plasma FN1 (dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. FN1 binds to cell surfaces through integrins and to various compounds including collagen, fibrin and heparin. It is involved in cell adhesion and migration processes including embryogenesis, wound healing, hemostasis, host defense, and metastasis. (PMID: 7276612; 12244123; 12847088)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Dev Cell; Vimentin intermediate filaments coordinate actin stress fibers and podosomes to determine the extracellular matrix degradation by macrophages; Authors - Xinyi Huang
rat	WB,IF	Int J Biol Macromol; A multifunctional polydopamine/genipin/alendronate nanoparticle licences fibrin hydrogels osteoinductive and immunomodulatory potencies for repairing bone defects; Authors - Wentao Shi
human	WB,IF	Biomed Pharmacother; Nintedanib prevents TGF-β2-induced epithelial-mesenchymal transition in retinal pigment epithelial cells; Authors - Yiwei Yin
mouse	IHC	Oxid Med Cell Longev; HBO1 as an Important Target for the Treatment of CCL4-Induced Liver Fibrosis and Aged-Related Liver Aging and Fibrosis; Authors - Baopeng Xing
mouse	IHC	Front Immunol; Integrated analysis reveals crosstalk between pyroptosis and immune regulation in renal fibrosis; Authors - Fengxia Bai
mouse	WB	Ecotoxicol Environ Saf; Alleviative effect of melatonin against the nephrotoxicity induced by cadmium exposure through regulating renal oxidative stress, inflammatory reaction, and fibrosis in a mouse model; Authors - Zhijie Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Udesh (Verified Customer) (04-19-2023)

Worked well for IP, band seen just below 250 kD (page ruler plus ladder). Lower band is light chain IgG

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: BM-MSCs

Fibronectin Antibody Western Blot,Immunoprecipitation validation (1:1000 dilution) in BM-MSCs (Cat no:66042-1-Ig)

FH

Ren Jie (Verified Customer) (07-11-2022)

Quite significant smear and non-specific binding

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human imortalized mammary fibroblast

Fibronectin Antibody Western Blot validation (1:1000 dilution) in Human imortalized mammary fibroblast (Cat no:66042-1-Ig)

FH

Deqiang (Verified Customer) (05-14-2020)

it works well on mouse embryo paraffin sections.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100

FH

Jihyun (Verified Customer) (05-07-2020)

There is no background signal. I can get a good one band in the WB. Great!

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse epithelial cell