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Fibronectin Monoclonal antibody

Fibronectin Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

1G10F9

Cat no : 66042-1-Ig

Synonyms

CIG, Cold insoluble globulin, ED B, Fibronectin, fibronectin 1, FINC, FN, FN1, FNZ, GFND, GFND2, LETS, MSF



Tested Applications

Positive WB detected inhuman plasma tissue, rat serum tissue, human brain tissue, human liver tissue
Positive IP detected inhuman plasma tissue
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells, human colon cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66042-1-Ig is Fibronectin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Fibronectin fusion protein Ag8016
Full Name fibronectin 1
Calculated molecular weight 2386 aa, 263 kDa
Observed molecular weight 263 kDa
GenBank accession numberBC005858
Gene symbol FN1
Gene ID (NCBI) 2335
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fibronectin 1 (FN1) is a high molecular weight glycoprotein which exists in both a soluble form in plasma (plasma FN1) and other body fluids and an insoluble form in the extracellular matrix (cellular FN1). Plasma FN1 (dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. FN1 binds to cell surfaces through integrins and to various compounds including collagen, fibrin and heparin. It is involved in cell adhesion and migration processes including embryogenesis, wound healing, hemostasis, host defense, and metastasis. (PMID: 7276612; 12244123; 12847088)

Protocols

Product Specific Protocols
WB protocol for Fibronectin antibody 66042-1-IgDownload protocol
IHC protocol for Fibronectin antibody 66042-1-IgDownload protocol
IF protocol for Fibronectin antibody 66042-1-IgDownload protocol
IP protocol for Fibronectin antibody 66042-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Oxid Med Cell Longev

HBO1 as an Important Target for the Treatment of CCL4-Induced Liver Fibrosis and Aged-Related Liver Aging and Fibrosis

Authors - Baopeng Xing
mouseWB,IF

Environ Pollut

Accumulation of polystyrene microplastics induces liver fibrosis by activating cGAS/STING pathway.

Authors - Rong Shen
humanWB

J Med Chem

Discovery of a Novel Small-Molecule Inhibitor Disrupting TRBP-Dicer Interaction against Hepatocellular Carcinoma via the Modulation of microRNA Biogenesis.

Authors - Ting Peng
humanWB

Am J Respir Cell Mol Biol

The Combined Effects of Circular RNA Methylation Promote Pulmonary Fibrosis.

Authors - Sha Wang
humanWB,IHC

Front Cell Dev Biol

Targeting MALT1 Suppresses the Malignant Progression of Colorectal Cancer via miR-375/miR-365a-3p/NF-κB Axis.

Authors - Rui Qian
human,mouseWB

Biochem Pharmacol

The ubiquitination of CKIP-1 mediated by Src aggravates diabetic renal fibrosis (original article)

Authors - Yan Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ren Jie (Verified Customer) (07-11-2022)

Quite significant smear and non-specific binding

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human imortalized mammary fibroblast
Fibronectin Antibody Western Blot validation (1:1000 dilution) in Human imortalized mammary fibroblast (Cat no:66042-1-Ig)
FH

Deqiang (Verified Customer) (05-14-2020)

it works well on mouse embryo paraffin sections.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
FH

Jihyun (Verified Customer) (05-07-2020)

There is no background signal. I can get a good one band in the WB. Great!

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse epithelial cell