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Fibronectin Antibody 12 Publications

Mouse Monoclonal| Catalog number: 66042-1-Ig |CloneNo.: 1G10F9

Featured Product KD/KO validated

2 customer reviews

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Con: 150 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
Application not tested? Please see our Guarantee
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.


Images See all

Fibronectin Antibody WB human plasma tissue 66042-1-Ig

human plasma were subjected to SDS PAGE followed by western blot with 66042-1-Ig( Fibronectin antibody at dilution of 1:6000 incubated at room temperature for 1.5 hours

Fibronectin Antibody IP human plasma tissue 66042-1-Ig

IP Result of anti-Fibronectin (IP:66042-1-Ig, 5ug; Detection:66042-1-Ig 1:500) with human plasma lysate 4000ug.

Fibronectin Antibody IHC human colon cancer tissue 66042-1-Ig

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66042-1-Ig( Fibronectin antibody) at dilution of 1:600 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Fibronectin Antibody IHC human colon cancer tissue 66042-1-Ig

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66042-1-Ig( Fibronectin antibody) at dilution of 1:600 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Fibronectin Antibody IF NIH/3T3 cells 66042-1-Ig

Immunofluorescent analysis of ( -20°C Ethanol ) fixed NIH/3T3 cells using 66042-1-Ig(Fibronectin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
human plasma tissue, rat serum tissue, human brain tissue, human liver tissue
Positive IP detected in:
human plasma tissue
Positive IHC detected in:
human colon cancer tissue, human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
NIH/3T3 cells
Recommended dilution:
WB : 1:2000-1:12000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:300-1:1200
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Fibronectin fusion protein Ag8016
Full name:
fibronectin 1
Calculated molecular weight:
2386aa,263 kDa
Observed molecular weight:
263 kDa
GenBank accession number:
BC005858
Gene ID (NCBI):
2335
Gene symbol
FN1
Synonyms
CIG, Cold insoluble globulin, ED B, Fibronectin, fibronectin 1, FINC, FN, FN1, FNZ, GFND, GFND2, LETS, MSF

Background

Fibronectin 1 (FN1) is a high molecular weight glycoprotein which exists in both a soluble form in plasma (plasma FN1) and other body fluids and an insoluble form in the extracellular matrix (cellular FN1). Plasma FN1 (dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. FN1 binds to cell surfaces through integrins and to various compounds including collagen, fibrin and heparin. It is involved in cell adhesion and migration processes including embryogenesis, wound healing, hemostasis, host defense, and metastasis. (PMID: 7276612; 12244123; 12847088)

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

Fibronectin Mouse Monoclonal antibody

Deqiang (Verified Customer), 05-14-2020
Applications
Immunofluorescence
Primary Antibody Dilution
1:100
No image submitted with review

No image submitted with review

Lot Number
10003852
it works well on mouse embryo paraffin sections.

Fibronectin Mouse Monoclonal antibody

Jihyun (Verified Customer), 05-07-2020
Applications
Western Blot
Primary Antibody Dilution
1:1000
No image submitted with review

No image submitted with review

Cell/Tissue Type
mouse epithelial cell
Lot Number
10003852
There is no background signal. I can get a good one band in the WB. Great!

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