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Fibronectin Monoclonal antibody

Fibronectin Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

1G10F9

Cat no : 66042-1-Ig

Synonyms

CIG, Cold insoluble globulin, ED B, Fibronectin, fibronectin 1, FINC, FN, FN1, FNZ, GFND, GFND2, LETS, MSF



Tested Applications

Positive WB detected inhuman plasma tissue, human plasma, rat serum, HuH-7 cells, HepG2 cells, MG-63 cells, U2OS cells
Positive IP detected inhuman plasma tissue
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells, human colon cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

66042-1-Ig targets Fibronectin in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Fibronectin fusion protein Ag8016
Full Name fibronectin 1
Calculated molecular weight 2386 aa, 263 kDa
Observed molecular weight 263 kDa
GenBank accession numberBC005858
Gene symbol FN1
Gene ID (NCBI) 2335
RRIDAB_11182385
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibronectin 1 (FN1) is a high molecular weight glycoprotein which exists in both a soluble form in plasma (plasma FN1) and other body fluids and an insoluble form in the extracellular matrix (cellular FN1). Plasma FN1 (dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. FN1 binds to cell surfaces through integrins and to various compounds including collagen, fibrin and heparin. It is involved in cell adhesion and migration processes including embryogenesis, wound healing, hemostasis, host defense, and metastasis. (PMID: 7276612; 12244123; 12847088)

Protocols

Product Specific Protocols
WB protocol for Fibronectin antibody 66042-1-IgDownload protocol
IHC protocol for Fibronectin antibody 66042-1-IgDownload protocol
IF protocol for Fibronectin antibody 66042-1-IgDownload protocol
IP protocol for Fibronectin antibody 66042-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IF

Int J Biol Macromol

A multifunctional polydopamine/genipin/alendronate nanoparticle licences fibrin hydrogels osteoinductive and immunomodulatory potencies for repairing bone defects

Authors - Wentao Shi
humanWB,IF

Biomed Pharmacother

Nintedanib prevents TGF-β2-induced epithelial-mesenchymal transition in retinal pigment epithelial cells

Authors - Yiwei Yin
mouseIHC

Oxid Med Cell Longev

HBO1 as an Important Target for the Treatment of CCL4-Induced Liver Fibrosis and Aged-Related Liver Aging and Fibrosis

Authors - Baopeng Xing
mouseWB

Ecotoxicol Environ Saf

Alleviative effect of melatonin against the nephrotoxicity induced by cadmium exposure through regulating renal oxidative stress, inflammatory reaction, and fibrosis in a mouse model

Authors - Zhijie Yang
mouseWB,IF

Environ Pollut

Accumulation of polystyrene microplastics induces liver fibrosis by activating cGAS/STING pathway.

Authors - Rong Shen
humanFC

Int J Mol Sci

Differential Angiogenic Potential of 3-Dimension Spheroid of HNSCC Cells in Mouse Xenograft.

Authors - So-Young Choi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Udesh (Verified Customer) (04-19-2023)

Worked well for IP, band seen just below 250 kD (page ruler plus ladder). Lower band is light chain IgG

  • Applications: Western Blot, Immunoprecipitation
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: BM-MSCs
Fibronectin Antibody Western Blot,Immunoprecipitation validation (1:1000 dilution) in BM-MSCs (Cat no:66042-1-Ig)
FH

Ren Jie (Verified Customer) (07-11-2022)

Quite significant smear and non-specific binding

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human imortalized mammary fibroblast
Fibronectin Antibody Western Blot validation (1:1000 dilution) in Human imortalized mammary fibroblast (Cat no:66042-1-Ig)
FH

Deqiang (Verified Customer) (05-14-2020)

it works well on mouse embryo paraffin sections.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
FH

Jihyun (Verified Customer) (05-07-2020)

There is no background signal. I can get a good one band in the WB. Great!

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse epithelial cell