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CoraLite®594-conjugated Fibronectin Monoclonal antibody
Fibronectin Monoclonal Antibody for FC (Intra), IF
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
IF, FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1G10F9
Cat no : CL594-66042
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | human colon cancer tissue, NIH/3T3 cells |
Positive FC detected in | HEK-293 cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
The immunogen of CL594-66042 is Fibronectin Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Fibronectin fusion protein Ag8016 |
Full Name | fibronectin 1 |
Calculated molecular weight | 2386 aa, 263 kDa |
Observed molecular weight | 263 kDa |
GenBank accession number | BC005858 |
Gene symbol | FN1 |
Gene ID (NCBI) | 2335 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Fibronectin 1 (FN1) is a high molecular weight glycoprotein which exists in both a soluble form in plasma (plasma FN1) and other body fluids and an insoluble form in the extracellular matrix (cellular FN1). Plasma FN1 (dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. FN1 binds to cell surfaces through integrins and to various compounds including collagen, fibrin and heparin. It is involved in cell adhesion and migration processes including embryogenesis, wound healing, hemostasis, host defense, and metastasis. (PMID: 7276612; 12244123; 12847088)
Protocols
Product Specific Protocols | |
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IF protocol for CL594 Fibronectin antibody CL594-66042 | Download protocol |
FC protocol for CL594 Fibronectin antibody CL594-66042 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |