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CoraLite® Plus 488-conjugated Fibronectin Monoclonal antibody

Fibronectin Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IHC, IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G10F9

Cat no : CL488-66042

Synonyms

CIG, Cold insoluble globulin, DKFZp686F10164, DKFZp686H0342, DKFZp686I1370, DKFZp686O13149, ED B, Fibronectin, fibronectin 1, FINC, FN, FN1, FNZ, GFND, GFND2, LETS, MSF



Tested Applications

Positive IF detected inNIH/3T3 cells, human colon cancer tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 1 publications below

Product Information

The immunogen of CL488-66042 is Fibronectin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Fibronectin fusion protein Ag8016
Full Name fibronectin 1
Calculated molecular weight 2386 aa, 263 kDa
Observed molecular weight 263 kDa
GenBank accession numberBC005858
Gene symbol FN1
Gene ID (NCBI) 2335
RRIDAB_2883236
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibronectin 1 (FN1) is a high molecular weight glycoprotein which exists in both a soluble form in plasma (plasma FN1) and other body fluids and an insoluble form in the extracellular matrix (cellular FN1). Plasma FN1 (dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. FN1 binds to cell surfaces through integrins and to various compounds including collagen, fibrin and heparin. It is involved in cell adhesion and migration processes including embryogenesis, wound healing, hemostasis, host defense, and metastasis. (PMID: 7276612; 12244123; 12847088)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Fibronectin antibody CL488-66042Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Front Pharmacol

Ginsenoside Rb1 Ameliorates Diabetic Arterial Stiffening via AMPK Pathway.

Authors - Xinyu Zhang