Validation Data Gallery
|Positive WB detected in||HT-29 cells, HEK-293 cells, human testis tissue, HeLa cells, mouse thymus tissue, mouse colon tissue, mouse testis tissue, mouse spleen tissue, MCF-7 cells, A431 cells, L02 cells|
|Positive IP detected in||L02 cells|
|Positive IHC detected in||human pancreas cancer tissue, human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
13147-1-AP targets FOXM1 in WB, RIP, IP, IHC, ChIP, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||FOXM1 fusion protein Ag3729|
|Full Name||forkhead box M 1|
|Calculated molecular weight||83 kDa|
|Observed molecular weight||110 kDa|
|GenBank accession number||BC006192|
|Gene ID (NCBI)||2305|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Forkhead box M1 (FOXM1), also named as HNF-3, HFH-11 or Trident, belongs to a superfamily of Fox transcription factors. FOXM1 is strongly associated with cell proliferation, it plays a important role in cell cycle progression by regulating both G1/S and G2/M phases, it is also involved in DNA damage repair, angiogenesis, apoptosis and tissue regeneration. Recently, crucial roles of this transcription factor were found in the development and progression of many cancers including colorectal, lung, prostate, liver and breast cancer. FOXM1 levels are significantly higher and associated with tumor grade in most human tumors, rendering it a potential target of cancer diagnosis and therapies. Catalog#13147-1-AP is a rabbit polyclonal antibody raised against C-terminal of human FOXM1. And this antibody can recognize three kinds of FOXM1, FOXM1-pp(~95-110kDa), FOXM1-B(isoform2,~83kDa) and FOXM1A(isoform4, ~90kDa). Sometimes a molecular weight of about 65 kDa can also be observed, which may be a degradation form of FOXM1 (PMID: 26547933).
Interferon-a potentiates anti-PD-1 efficacy by remodeling glucose metabolism in the hepatocellular carcinoma microenvironment.
Vagus-macrophage-hepatocyte link promotes post-injury liver regeneration and whole-body survival through hepatic FoxM1 activation.
FoxM1 repression during human aging leads to mitotic decline and aneuploidy-driven full senescence.
circTP63 functions as a ceRNA to promote lung squamous cell carcinoma progression by upregulating FOXM1.
POH1 deubiquitylates and stabilizes E2F1 to promote tumour formation.
LncBRM initiates YAP1 signalling activation to drive self-renewal of liver cancer stem cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kausik (Verified Customer) (02-21-2022)
The antibody worked well with immunoprecipitation and ChIP experiments and we had acceptable results with immunoblotting.
Iram (Verified Customer) (09-17-2020)
FOXM1 antibody giving a very clear bands.
Terri (Verified Customer) (11-05-2019)
This antibody distinguishes multiple isoforms of FOXM1.