• Featured Product
  • KD/KO Validated

FOXM1 Polyclonal antibody

FOXM1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, chIP, ELISA

Conjugate

Unconjugated

Cat no : 13147-1-AP

Synonyms

FKHL16, forkhead box M1, FOXM1, FOXM1B, HFH 11, HFH11, HNF 3, HNF 3/fork head homolog 11, INS 1, M phase phosphoprotein 2, MPHOSPH2, MPP 2, MPP2, PIG29, TGT3, Transcription factor Trident, TRIDENT, WIN



Tested Applications

Positive WB detected inmouse thymus tissue, L02 cells, mouse colon tissue, human testis tissue, mouse testis tissue, mouse spleen tissue, HeLa cells
Positive IP detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

13147-1-AP targets FOXM1 in WB, IP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FOXM1 fusion protein Ag3729
Full Name forkhead box M 1
Calculated molecular weight 83 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC006192
Gene symbol FOXM1
Gene ID (NCBI) 2305
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Forkhead box M1 (FOXM1), also named HNF-3, HFH-11 or Trident, belongs to a superfamily of Fox transcription factors. FOXM1 is strongly associated with cell proliferation, it plays a important role in cell cycle progression by regulating both G1/S and G2/M phases, it is also involved in DNA damage repair, angiogenesis, apoptosis and tissue regeneration. Recently, crucial roles of this transcription factor were found in the development and progression of many cancers including colorectal, lung, prostate, liver and breast cancer. FOXM1 levels are significantly higher and associated with tumor grade in most human tumors, rendering it a potential target of cancer diagnosis and therapies. Catalog#13147-1-AP is rabbit polyclonal antibody raised against C-terminal of human FOXM1. And this antibody can recognize three kinds of FOXM1,FOXM1-pp(~95-110kDa), FOXM1-B(isoform2,~83kDa) and FOXM1A(isoform4, ~90kDa)

Protocols

Product Specific Protocols
WB protocol for FOXM1 antibody 13147-1-APDownload protocol
IP protocol for FOXM1 antibody 13147-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Eur J Pharmacol

Silibinin-induced glioma cell apoptosis by PI3K-mediated but Akt-independent downregulation of FoxM1 expression.

Authors - Mingjie Zhang
humanWB

Biomed Res Int

Integrated Bioinformatics Analysis of Master Regulators in Anaplastic Thyroid Carcinoma.

Authors - Zongfu Pan
humanWB

Breast Cancer Res

Dynamically decreased miR-671-5p expression is associated with oncogenic transformation and radiochemoresistance in breast cancer.

Authors - Xiaohui Tan
humanWB

Biomolecules

Suppression of Hepatocellular Carcinoma Progression through FOXM1 and EMT Inhibition via Hydroxygenkwanin-Induced miR-320a Expression.

Authors - Li-Fang Chou
  • KD Validated
humanWB,IF

Oncotarget

miR-671-5p inhibits epithelial-to-mesenchymal transition by downregulating FOXM1 expression in breast cancer.

Authors - Xiaohui Tan
mouseWB

Cell Death Dis

A Cdh1-FoxM1-Apc axis controls muscle development and regeneration.

Authors - Zhe Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Iram (Verified Customer) (09-17-2020)

FOXM1 antibody giving a very clear bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000 for western
  • Cell Tissue Type: Human colon cancer
FH

Terri (Verified Customer) (11-05-2019)

This antibody distinguishes multiple isoforms of FOXM1.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: human mesothelioma and ovarian cell lines