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  • KD/KO Validated

FOXM1 Polyclonal antibody

FOXM1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB,IP,IHC,ELISA

Conjugate

Unconjugated

Cat no : 13147-1-AP

Synonyms

FKHL16, forkhead box M1, FOXM1, FOXM1B, HFH 11, HFH11, HNF 3, HNF 3/fork head homolog 11, INS 1, M phase phosphoprotein 2, MPHOSPH2, MPP 2, MPP2, PIG29, TGT3, Transcription factor Trident, TRIDENT, WIN



Tested Applications

Positive WB detected inHT-29 cells, HeLa cells, human testis tissue, mouse thymus tissue, mouse colon tissue, mouse testis tissue, HEK-293 cells, mouse spleen tissue, L02 cells, MCF-7 cells, A431 cells
Positive IP detected inL02 cells
Positive IHC detected inhuman pancreas cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13147-1-AP targets FOXM1 in WB,IP,IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FOXM1 fusion protein Ag3729
Full Name forkhead box M 1
Calculated Molecular Weight 83 kDa
Observed Molecular Weight 84-110 kDa
GenBank Accession NumberBC006192
Gene Symbol FOXM1
Gene ID (NCBI) 2305
RRIDAB_2106213
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Forkhead box M1 (FOXM1), also named as HNF-3, HFH-11 or Trident, belongs to a superfamily of Fox transcription factors. FOXM1 is strongly associated with cell proliferation, it plays a important role in cell cycle progression by regulating both G1/S and G2/M phases, it is also involved in DNA damage repair, angiogenesis, apoptosis and tissue regeneration. Recently, crucial roles of this transcription factor were found in the development and progression of many cancers including colorectal, lung, prostate, liver and breast cancer. FOXM1 levels are significantly higher and associated with tumor grade in most human tumors, rendering it a potential target of cancer diagnosis and therapies. Catalog#13147-1-AP is a rabbit polyclonal antibody raised against C-terminal of human FOXM1. And this antibody can recognize three kinds of FOXM1, FOXM1-pp(~95-110kDa), FOXM1-B(isoform2,~83kDa) and FOXM1A(isoform4, ~90kDa). Sometimes a molecular weight of about 65 kDa can also be observed, which may be a degradation form of FOXM1 (PMID: 26547933).

Protocols

Product Specific Protocols
WB protocol for FOXM1 antibody 13147-1-APDownload protocol
IHC protocol for FOXM1 antibody 13147-1-APDownload protocol
IP protocol for FOXM1 antibody 13147-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cancer Discov

Interferon-a potentiates anti-PD-1 efficacy by remodeling glucose metabolism in the hepatocellular carcinoma microenvironment.

Authors - Bo Hu
  • KD Validated
humanWB

Nat Commun

K235 acetylation couples with PSPC1 to regulate the m6A demethylation activity of ALKBH5 and tumorigenesis

Authors - Xiao-Lan Zhang
mouseWB

Acta Pharm Sin B

Constitutive androstane receptor induced-hepatomegaly and liver regeneration is partially via yes-associated protein activation.

Authors - Yue Gao
mouseWB

Nat Commun

Vagus-macrophage-hepatocyte link promotes post-injury liver regeneration and whole-body survival through hepatic FoxM1 activation.

Authors - Tomohito Izumi
humanWB

Nat Commun

FoxM1 repression during human aging leads to mitotic decline and aneuploidy-driven full senescence.

Authors - Joana Catarina Macedo
humanWB

Nat Commun

circTP63 functions as a ceRNA to promote lung squamous cell carcinoma progression by upregulating FOXM1.

Authors - Zhuoan Cheng
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kausik (Verified Customer) (02-21-2022)

The antibody worked well with immunoprecipitation and ChIP experiments and we had acceptable results with immunoblotting.

  • Applications: Western Blot, Immunoprecipitation, ChIP
  • Cell Tissue Type: human cancer-derived cell lines
FH

Iram (Verified Customer) (09-17-2020)

FOXM1 antibody giving a very clear bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000 for western
  • Cell Tissue Type: Human colon cancer
FH

Terri (Verified Customer) (11-05-2019)

This antibody distinguishes multiple isoforms of FOXM1.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: human mesothelioma and ovarian cell lines