FOXP4 Polyclonal antibody

FOXP4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29311-1-AP

Synonyms

FKHLA, FLJ40908, FLJ44184, forkhead box P4, Forkhead box protein P4, FOXP4, hFKHLA

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Validation Data Gallery

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  WB analysis using 29311-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29311-1-AP (FOXP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse ovary using 29311-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 29311-1-AP (FOXP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 29311-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 29311-1-AP (FOXP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 29311-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 29311-1-AP (FOXP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Caco-2 cells, PC-3 cells
• Positive IHC detected in: mouse ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29311-1-AP targets FOXP4 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FOXP4 fusion protein Ag29953
• Full Name: forkhead box P4
• Calculated Molecular Weight: 678 aa, 73 kDa
• Observed Molecular Weight: 73 kDa
• GenBank Accession Number: BC052803
• Gene Symbol: FOXP4
• Gene ID (NCBI): 116113
• RRID: AB_2918279
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FOXP4, also named as FKHLA, belongs to the FOX family. FOXP4 has been implicated in diverse biological processes in tumor initiation and progression. It has been demonstrated that FOXP4 is significantly upregulated in breast cancer, hepatocellular carcinoma and oral squamous cell carcinoma (PMID: 34590150). FOXP4 has 3 isoforms with the molecular mass of 72-73 kDa.