Tested Applications
| Positive WB detected in | HeLa cells, SH-SY5Y cells, human brain tissue, NIH/3T3 cells, PC-3 cells |
| Positive IHC detected in | human prostate cancer tissue, mouse liver tissue, mouse ovary tissue, human prostate cancer tissue, human rectal cancer tissue, rat liver tissue, rat ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11503-1-AP targets FRS2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2052 Product name: Recombinant human FRS2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-302 aa of BC021562 Sequence: MGSCCSCPDKDTVPDNHRNKFKVINVDDDGNELGSGIMELTDTELILYTRKRDSVKWHYLCLRRYGYDSNLFSFESGRRCQTGQGIFAFKCARAEELFNMLQEIMQNNSINVVEEPVVERNNHQTELEVPRTPRTPTTPGFAAQNLPNGYPRYPSFGDASSHPSSRHPSVGSARLPSVGEESTHPLLVAEEQVHTYVNTTGVQEERKNRTSVHVPLEARVSNAESSTPKEEPSSIEDRDPQILLEPEGVKFVLGPTPVQKQLMEKEKLEQLGRDQVSGSGANNTEWDTGYDSDERRDAPSVN Predict reactive species |
| Full Name | fibroblast growth factor receptor substrate 2 |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 68 kDa |
| GenBank Accession Number | BC021562 |
| Gene Symbol | FRS2 |
| Gene ID (NCBI) | 10818 |
| RRID | AB_2262942 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WU20 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Fibroblast growth factor (FGF) receptor substrate 2 (FRS2) has an alternative name as SNT-1, it is an adapter protein that links activated FGR and NGF receptors to downstream signaling pathways. FGF receptor substrates (FRS2 and FRS3) are key adaptor proteins that mediate FGF-FGFR signalling in benign as well as malignant tissue. FRS2 is a 508 amino-acid protein,which is phosphorylated on tyrosine residues. The molecular weight of non-phosphorylated FRS2 is 57-68 kDa, but phosphorylated FRS2 is 80-90 kDa. Phosphorylation of FRS2 is associated with activation of a number of MAP kinases. Allele-specific regulation of FGFR2 mRNA expression with a mildly increased breast cancer risk has been reported.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FRS2 antibody 11503-1-AP | Download protocol |
| IHC protocol for FRS2 antibody 11503-1-AP | Download protocol |
| WB protocol for FRS2 antibody 11503-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FRS2 Polyclonal antibody (Cat# 11503-1-AP) has 13 citations. These appear in journals including Cancer Research, Cell Reports, Cell Death & Disease, Journal of Medicinal Chemistry, Breast Cancer Research, Cells, Genes, Chromosomes & Cancer, Physiological Reports, Journal of Advanced Research, Cell Immunology, and bioRxiv. Research fields span FGFR/FGF signaling, cancer biology (liposarcoma, breast cancer, lung adenocarcinoma, endometrial cancer), autophagy, integrin biology, and wound healing.
| Species | Application | Title |
|---|---|---|
Cancer Res Transcriptomics and transposon mutagenesis identify multiple mechanisms of resistance to the FGFR inhibitor AZD4547. | ||
Cancer Res Amplification of FRS2 and activation of FGFR/FRS2 signaling pathway in high-grade liposarcoma. | ||
J Adv Res Synergistic Antitumor Activity and Neuroprotective Effects of FGF1/FGFR Inhibition with Oxaliplatin Chemotherapy. | ||
Cell Death Dis Inhibition of autophagy potentiates the cytotoxicity of the irreversible FGFR1-4 inhibitor FIIN-2 on lung adenocarcinoma | ||
Cell Rep IMPDH2 dephosphorylation under FGFR signaling promotes S-phase progression and tumor growth | ||
J Med Chem Design, Synthesis, and Biological Evaluation of Aminoindazole Derivatives as Highly Selective Covalent Inhibitors of Wild-Type and Gatekeeper Mutant FGFR4. |
Reviews
What customers say
Both reviewers used this antibody for Western Blot at a 1:1000 dilution. One user (4/5) reported it works well on liver tissue. The other (5/5) found it very effective for detecting endogenous protein with and without RNAi, and noted complete overlap with phospho-FRS2 signal after cell stimulation in HEK293, PC12, and AR42J cell lines. Overall, users are satisfied with its performance for WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
MALLIKARJUNA (Verified Customer) (11-26-2025) | woks ok for Wb
|
Barbara (Verified Customer) (08-01-2025) | very good for endogenous protein, +/- RNAi, total overlap with phosphoFRS2 after stimulation of the cells.
|
