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Follistatin Monoclonal antibody

Follistatin Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b


human, mouse and More (2)







Cat no : 60060-1-Ig


Activin binding protein, follistatin, FS, FST

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Raji cells, U2OS cells, SKOV-3 cells, A549 cells, NCI-H1299 cells, Calu-3 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60060-1-Ig targets Follistatin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, camel
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Follistatin fusion protein Ag0775
Full Name follistatin
Calculated Molecular Weight 33 aa, 7 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC004107
Gene Symbol FST
Gene ID (NCBI) 10468
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Follistatin (FST) is a member of the tissue growth factor β family and is a secreted glycoprotein that antagonizes many members of the family, including activin A, growth differentiation factor11 , and myostatin. It binds activin A with high affinity and whose expression can be induced by activin A and several other pro-inflammatory cytokines. Activin A-follistatin complexes are biologically inactive and bind to cell surface heparan sulphate-containing proteoglycans for internalisation and degradation. It has also been found to play a significant role in the management of skeletal muscle size and mass. It also has important roles in early embryonic development, differentiation of ovarian granulosa cells, liver fibrosis and polycystic ovarian syndrome.


Product Specific Protocols
WB protocol for Follistatin antibody 60060-1-IgDownload protocol
IHC protocol for Follistatin antibody 60060-1-IgDownload protocol
IF protocol for Follistatin antibody 60060-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Tubule-Derived Follistatin Is Increased in the Urine of Rats with Renal Ischemia and Reflects the Severity of Acute Tubular Damage

Authors - Izumi Nagayama

Antioxid Redox Signal

Follistatin Protects against Glomerular Mesangial Cell Apoptosis and Oxidative Stress to Ameliorate Chronic Kidney Disease.

Authors - Neel Mehta

Front Pharmacol

Follistatin Attenuates Myocardial Fibrosis in Diabetic Cardiomyopathy via the TGF-β-Smad3 Pathway.

Authors - Yinhui Wang

Fertil Steril

Myostatin, follistatin and activin type II receptors are highly expressed in adenomyosis.

Authors - Patrizia Carrarelli


Development of a gene doping detection method to detect overexpressed human follistatin using an adenovirus vector in mice.

Authors - Koki Yanazawa

Animals (Basel)

Screening and Identification of Differential Ovarian Proteins before and after Induced Ovulation via Seminal Plasma in Bactrian Camels.

Authors - Qi Wang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Jennifer (Verified Customer) (07-17-2023)

I am not sure what this antibody targets, but I am sure it is not Follistatin. This is a glycosylated protein that appears as a smear at ~40 - 50 kDa. However, with this antibody there is a single band at ~ 35 kDa. We have validated several FST antibodies using knock-down and overexpression cell lines so we know what to expect.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5,000
  • Cell Tissue Type: HNSCC cell lines