FUCA1 Polyclonal antibody

• 

  WB analysis of mouse liver using 16420-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16420-1-AP (FUCA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 16420-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16420-1-AP (FUCA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 16420-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16420-1-AP (FUCA1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 16420-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16420-1-AP (FUCA1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse brain using 16420-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16420-1-AP (FUCA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16420-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16420-1-AP (FUCA1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16420-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16420-1-AP (FUCA1 antibody) at dilution of 1:50 (under 40x lens).

FUCA1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16420-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

Alpha-L-fucosidase 1, Alpha-L-fucosidase I, Alpha-L-fucoside fucohydrolase 1, EC:3.2.1.51, FUCA

---

Tested Applications

• Positive WB detected in: mouse liver tissue, COLO 320 cells
• Positive IHC detected in: human testis tissue, human liver cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 6 publications below
• IF: See 1 publications below

Product Information

16420-1-AP targets FUCA1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9612

  Product name: Recombinant human FUCA1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 113-461 aa of BC017338

  Sequence: EEWADLFQAAGAKYVVLTTKHHEGFTNWPSPVSWNWNSKDVGPHRDLVGELGTALRKRNIRYGLYHSLLEWFHPLYLLDKKNGFKTQHFVSAKTMPELYDLVNSYKPDLIWSDGEWECPDTYWNSTNFLSWLYNDSPVKDEVVVNDRWGQNCSCHHGGYYNCEDKFKPQSLPDHKWEMCTSIDKFSWGYRRDMALSDVTEESEIISELVQTVSLGGNYLLNIGPTKDGLIVPIFQERLLAVGKWLSINGEAIYASKPWRVQWEKNTTSVWYTSKGSAVYAIFLHWPENGVLNLESPITTSTTKITMLGIQGDLKWSTDPDKGLFISLPQLPPSAVPAEFAWTIKLTGVK

• Full Name: fucosidase, alpha-L- 1, tissue
• Calculated Molecular Weight: 461 aa, 53 kDa
• Observed Molecular Weight: 50-56 kDa
• GenBank Accession Number: BC017338
• Gene Symbol: FUCA1
• Gene ID (NCBI): 2517
• RRID: AB_2294111
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P04066
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FUCA1(a-l-Fucosidase) is a lysosomal glycosidase which is ubiquitous in eukaryotic cells. This enzyme is usually found as a soluble component of the lysosome and functions as an acid hydrolase in the degradation of numerous and diverse fucoglycoconjugates.The mammalian a-l-fucosidases are relatively large, multisubunit glycoproteins which exist in multiple molecular isoforms.This enzyme may have a role in sperm-egg interactions in the reproductive tract of the female rat. There is a precursor 54 kDa form of rat sperm fucosidase which is processed to a mature 52 kDa form of the enzyme(PMID:8836125). This protein from rat epididymis is a tetramer of 210-220 kDa, made up by two pairs of subunits of 47 and 60 kDa, and the one from human seminal plasma exists in different oligomeric forms, that is, as a dimer, as a tetramer, and as a hexamer made up of a single subunit of 56-57 kDa(PMID:16736526).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	Oncotarget; Reduced expression of α-L-Fucosidase-1 (FUCA-1) predicts recurrence and shorter cancer specific survival in luminal B LN+ breast cancer patients.; Authors - Serena Bonin
human	WB,IHC	Oncotarget; Human a-L-fucosidase-1 attenuates the invasive properties of thyroid cancer.; Authors - Giancarlo Vecchio
human	WB,IHC	Gene; Potential predictive value of immune-related genes FUCA1 and NCKAP1L for osteosarcoma metastasis; Authors - Xuan Wang
human	WB	Cancer Genomics Proteomics; High Resolution Proteomic Analysis of the Cervical Cancer Cell Lines Secretome Documents Deregulation of Multiple Proteases.; Authors - Kalliopi I Pappa
human	IHC	Pathol Int; Alpha-L-fucosidase-1 is a diagnostic marker that distinguishes mucoepidermoid carcinoma from squamous cell carcinoma.; Authors - Shoko Ishida
human	IHC	Oncotarget; Proteomics analysis of bladder cancer invasion: Targeting EIF3D for therapeutic intervention.; Authors - Agnieszka Latosinska