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FUS/TLS Polyclonal antibody
FUS/TLS Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 11570-1-AP
|Positive WB detected in||HEK-293 cells, HeLa cells, Jurkat cells, HL-60 cells, K-562 cells, SMMC-7721 cells, human kidney tissue, HepG2 cells, mouse brain tissue|
|Positive IP detected in||K-562 cells|
|Positive IHC detected in||human breast cancer tissue, rat cerebellum tissue, mouse cerebellum tissue, mouse brain tissue, human ovary tumor tissue, rat brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11570-1-AP targets FUS/TLS in WB, RIP, IP, IHC, IF, FC, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||FUS/TLS fusion protein Ag2150|
|Full Name||fusion (involved in t(12;16) in malignant liposarcoma)|
|Calculated molecular weight||75 kDa|
|Observed molecular weight||68-75 kDa|
|GenBank accession number||BC026062|
|Gene ID (NCBI)||2521|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
FUS (also named TLS and POMp75) belongs to the RRM TET family. FUS may play a role in the maintenance of genomic integrity; it binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. FUS is also an RNA-binding protein, and its links to neurodegenerative disease proffer the intriguing possibility that altered RNA metabolism or RNA processing may underlie or contribute to neuron degeneration[PMID: 22640227]. FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) and is implicated in certain forms of amyotrophic lateral sclerosis (ALS) and frontotemporal dementias (FTDs) such as frontotemporal lobar dementia with ubiquitin inclusions (FTLD-U)[PMID: 22640227]. This antibody is a rabbit polyclonal antibody raised against an internal region of human FUS.
|Product Specific Protocols|
|WB protocol for FUS/TLS antibody 11570-1-AP||Download protocol|
|IHC protocol for FUS/TLS antibody 11570-1-AP||Download protocol|
|IF protocol for FUS/TLS antibody 11570-1-AP||Download protocol|
|IP protocol for FUS/TLS antibody 11570-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Exp Clin Cancer Res
FUS/circ_002136/miR-138-5p/SOX13 feedback loop regulates angiogenesis in Glioma.
Hum Mol Genet
Quantitative proteomics identifies proteins that resist translational repression and become dysregulated in ALS-FUS.
LINC00473 mediates cyclin D1 expression through a balance between activation and repression signals in breast cancer cells.
Acta Neuropathol Commun
Restoration of histone acetylation ameliorates disease and metabolic abnormalities in a FUS mouse model.
Acta Neuropathol Commun
Phosphorylated TDP-43 (pTDP-43) aggregates in the axial skeletal muscle of patients with sporadic and familial amyotrophic lateral sclerosis.
Antiviral Immune Response as a Trigger of FUS Proteinopathy in Amyotrophic Lateral Sclerosis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
shashirekha (Verified Customer) (12-23-2020)
Used for immunopreciptation at 1:1000 dilution. Works very well
H (Verified Customer) (04-06-2020)
The antibody worked well for HCT116 cell line. Nuclear cytosolic fractionation clearly showed that FUS is dominantly in nucleus, and sub-fraction is present in cytosol.
Karthik (Verified Customer) (04-24-2019)
Magenta- FUSBlue- MAP2FUS staining consistent obtained with this antibody is consistent with literature
Yen-Chen (Verified Customer) (12-03-2018)