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FUS/TLS Polyclonal antibody
FUS/TLS Polyclonal Antibody for FC, IF, IP, WB, ELISA
Cat no : 11570-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, HeLa cells, mouse brain tissue, HepG2 cells, SH-SY5Y cells|
|Positive IP detected in||K-562 cells|
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11570-1-AP targets FUS/TLS in WB, RIP, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||FUS/TLS fusion protein Ag2150|
|Full Name||fusion (involved in t(12;16) in malignant liposarcoma)|
|Calculated molecular weight||75 kDa|
|Observed molecular weight||68-75 kDa|
|GenBank accession number||BC026062|
|Gene ID (NCBI)||2521|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
FUS (also named TLS and POMp75) belongs to the RRM TET family. FUS may play a role in the maintenance of genomic integrity; it binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. FUS is also an RNA-binding protein, and its links to neurodegenerative disease proffer the intriguing possibility that altered RNA metabolism or RNA processing may underlie or contribute to neuron degeneration[PMID: 22640227]. FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) and is implicated in certain forms of amyotrophic lateral sclerosis (ALS) and frontotemporal dementias (FTDs) such as frontotemporal lobar dementia with ubiquitin inclusions (FTLD-U)[PMID: 22640227]. This antibody is a rabbit polyclonal antibody raised against an internal region of human FUS.
|Product Specific Protocols|
|WB protocol for FUS/TLS antibody 11570-1-AP||Download protocol|
|IHC protocol for FUS/TLS antibody 11570-1-AP||Download protocol|
|IF protocol for FUS/TLS antibody 11570-1-AP||Download protocol|
|IP protocol for FUS/TLS antibody 11570-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Divergent transcriptional regulation of astrocyte reactivity across disorders.
Mutations in UBQLN2 cause dominant X-linked juvenile and adult-onset ALS and ALS/dementia.
Antisense oligonucleotide silencing of FUS expression as a therapeutic approach in amyotrophic lateral sclerosis.
Nuclear-Import Receptors Reverse Aberrant Phase Transitions of RNA-Binding Proteins with Prion-like Domains.
NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.
FUS-mediated regulation of acetylcholine receptor transcription at neuromuscular junctions is compromised in amyotrophic lateral sclerosis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
shashirekha (Verified Customer) (12-23-2020)
Used for immunopreciptation at 1:1000 dilution. Works very well
H (Verified Customer) (04-06-2020)
The antibody worked well for HCT116 cell line. Nuclear cytosolic fractionation clearly showed that FUS is dominantly in nucleus, and sub-fraction is present in cytosol.
Karthik (Verified Customer) (04-24-2019)
Magenta- FUSBlue- MAP2FUS staining consistent obtained with this antibody is consistent with literature
Yen-Chen (Verified Customer) (12-03-2018)