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FUS/TLS Polyclonal antibody
FUS/TLS Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, RIP, IP, IHC, IF, FC, CoIP, chIP, ELISA
Conjugate
Unconjugated
Cat no : 11570-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, Jurkat cells, HL-60 cells, K-562 cells, SMMC-7721 cells, human kidney tissue, HepG2 cells, mouse brain tissue |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human breast cancer tissue, rat cerebellum tissue, mouse cerebellum tissue, mouse brain tissue, human ovary tumor tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 8 publications below |
| WB | See 37 publications below |
| IHC | See 25 publications below |
| IF | See 33 publications below |
| CoIP | See 1 publications below |
| ChIP | See 1 publications below |
| RIP | See 2 publications below |
Product Information
11570-1-AP targets FUS/TLS in WB, RIP, IP, IHC, IF, FC, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FUS/TLS fusion protein Ag2150 |
| Full Name | fusion (involved in t(12;16) in malignant liposarcoma) |
| Calculated molecular weight | 75 kDa |
| Observed molecular weight | 68-75 kDa |
| GenBank accession number | BC026062 |
| Gene symbol | FUS |
| Gene ID (NCBI) | 2521 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FUS (also named TLS and POMp75) belongs to the RRM TET family. FUS may play a role in the maintenance of genomic integrity; it binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. FUS is also an RNA-binding protein, and its links to neurodegenerative disease proffer the intriguing possibility that altered RNA metabolism or RNA processing may underlie or contribute to neuron degeneration[PMID: 22640227]. FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) and is implicated in certain forms of amyotrophic lateral sclerosis (ALS) and frontotemporal dementias (FTDs) such as frontotemporal lobar dementia with ubiquitin inclusions (FTLD-U)[PMID: 22640227]. This antibody is a rabbit polyclonal antibody raised against an internal region of human FUS.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FUS/TLS antibody 11570-1-AP | Download protocol |
| IHC protocol for FUS/TLS antibody 11570-1-AP | Download protocol |
| IF protocol for FUS/TLS antibody 11570-1-AP | Download protocol |
| IP protocol for FUS/TLS antibody 11570-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res FUS/circ_002136/miR-138-5p/SOX13 feedback loop regulates angiogenesis in Glioma. | ||
Hum Mol Genet Quantitative proteomics identifies proteins that resist translational repression and become dysregulated in ALS-FUS. | ||
FEBS Lett LINC00473 mediates cyclin D1 expression through a balance between activation and repression signals in breast cancer cells. | ||
Acta Neuropathol Commun Restoration of histone acetylation ameliorates disease and metabolic abnormalities in a FUS mouse model. | ||
Acta Neuropathol Commun Phosphorylated TDP-43 (pTDP-43) aggregates in the axial skeletal muscle of patients with sporadic and familial amyotrophic lateral sclerosis. | ||
Cell Rep Antiviral Immune Response as a Trigger of FUS Proteinopathy in Amyotrophic Lateral Sclerosis.
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Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH shashirekha (Verified Customer) (12-23-2020) | Used for immunopreciptation at 1:1000 dilution. Works very well
 |
FH H (Verified Customer) (04-06-2020) | The antibody worked well for HCT116 cell line. Nuclear cytosolic fractionation clearly showed that FUS is dominantly in nucleus, and sub-fraction is present in cytosol.
 |
FH Karthik (Verified Customer) (04-24-2019) | Magenta- FUSBlue- MAP2FUS staining consistent obtained with this antibody is consistent with literature
|
FH Yen-Chen (Verified Customer) (12-03-2018) |
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