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FUS/TLS Polyclonal antibody
FUS/TLS Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, RIP, IP, IHC, IF, FC, CoIP, chIP, ELISA
Conjugate
Unconjugated
Cat no : 11570-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HeLa cells, Jurkat cells, HL-60 cells, K-562 cells, SMMC-7721 cells, human kidney tissue, HepG2 cells, mouse brain tissue |
Positive IP detected in | K-562 cells |
Positive IHC detected in | human breast cancer tissue, rat cerebellum tissue, mouse cerebellum tissue, mouse brain tissue, human ovary tumor tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 8 publications below |
WB | See 44 publications below |
IHC | See 28 publications below |
IF | See 37 publications below |
CoIP | See 1 publications below |
ChIP | See 1 publications below |
RIP | See 2 publications below |
Product Information
11570-1-AP targets FUS/TLS in WB, RIP, IP, IHC, IF, FC, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | FUS/TLS fusion protein Ag2150 |
Full Name | fusion (involved in t(12;16) in malignant liposarcoma) |
Calculated molecular weight | 75 kDa |
Observed molecular weight | 68-75 kDa |
GenBank accession number | BC026062 |
Gene symbol | FUS |
Gene ID (NCBI) | 2521 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FUS (also named TLS and POMp75) belongs to the RRM TET family. FUS may play a role in the maintenance of genomic integrity; it binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. FUS is also an RNA-binding protein, and its links to neurodegenerative disease proffer the intriguing possibility that altered RNA metabolism or RNA processing may underlie or contribute to neuron degeneration[PMID: 22640227]. FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) and is implicated in certain forms of amyotrophic lateral sclerosis (ALS) and frontotemporal dementias (FTDs) such as frontotemporal lobar dementia with ubiquitin inclusions (FTLD-U)[PMID: 22640227]. This antibody is a rabbit polyclonal antibody raised against an internal region of human FUS.
Protocols
Product Specific Protocols | |
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WB protocol for FUS/TLS antibody 11570-1-AP | Download protocol |
IHC protocol for FUS/TLS antibody 11570-1-AP | Download protocol |
IF protocol for FUS/TLS antibody 11570-1-AP | Download protocol |
IP protocol for FUS/TLS antibody 11570-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Exp Clin Cancer Res FUS/circ_002136/miR-138-5p/SOX13 feedback loop regulates angiogenesis in Glioma. | ||
Hum Mol Genet Quantitative proteomics identifies proteins that resist translational repression and become dysregulated in ALS-FUS. | ||
FEBS Lett LINC00473 mediates cyclin D1 expression through a balance between activation and repression signals in breast cancer cells. | ||
Acta Neuropathol Commun Restoration of histone acetylation ameliorates disease and metabolic abnormalities in a FUS mouse model. | ||
Acta Neuropathol Commun Phosphorylated TDP-43 (pTDP-43) aggregates in the axial skeletal muscle of patients with sporadic and familial amyotrophic lateral sclerosis. | ||
Cell Rep Antiviral Immune Response as a Trigger of FUS Proteinopathy in Amyotrophic Lateral Sclerosis.
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Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH shashirekha (Verified Customer) (12-23-2020) | Used for immunopreciptation at 1:1000 dilution. Works very well
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FH H (Verified Customer) (04-06-2020) | The antibody worked well for HCT116 cell line. Nuclear cytosolic fractionation clearly showed that FUS is dominantly in nucleus, and sub-fraction is present in cytosol.
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FH Karthik (Verified Customer) (04-24-2019) | Magenta- FUSBlue- MAP2FUS staining consistent obtained with this antibody is consistent with literature
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FH Yen-Chen (Verified Customer) (12-03-2018) |
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