FUS/TLS Polyclonal antibody

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  WB analysis of HEK-293 using 11570-1-AP

  WB result of FUS antibody (11570-1-AP, 1:5000) with si-Control and si-FUS transfected HEK 293 cells.

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  WB analysis of HeLa using 11570-1-AP

  HeLa (WT and FUS KO) lysates prepared with RIPA buffer, 30 μg protein loaded. 11570-1-AP incubated at 1:5000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  WB analysis using 11570-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11570-1-AP (FUS/TLS antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 11570-1-AP

  IP result of anti-FUS/TLS (IP:11570-1-AP, 4ug; Detection:11570-1-AP 1:2000) with K-562 cells lysate 1760 ug.

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  IHC staining of mouse brain using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11570-1-AP (FUS/TLS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11570-1-AP (FUS/TLS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11570-1-AP (FUS/TLS Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11570-1-AP (FUS/TLS Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human ovary tumor using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11570-1-AP (FUS/TLS Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human ovary tumor using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11570-1-AP (FUS/TLS Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of rat brain using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11570-1-AP (FUS/TLS antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11570-1-AP (FUS/TLS antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse liver using 11570-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using FUS/TLS antibody (11570-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse colon using 11570-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using FUS/TLS antibody (11570-1-AP) at dilution of 1:200, CoraLite®594 smooth muscle actin antibody (CL594-14395, red), CD45 antibody (80297-1-RR, Clone: 6O19, orange), E-cadherin antibody (20874-1-AP, Magenta).

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  IF Staining of rat brain using 11570-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using FUS/TLS antibody (11570-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HeLa using 11570-1-AP

  HeLa WT cells (yellow outline) and FUS KO cells (red outline) labelled with a green or a far-red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 11570-1-AP at 1:2000 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IF Staining of HepG2 using 11570-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FUS/TLS antibody (11570-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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  IF Staining of HeLa using 11570-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FUS/TLS antibody (11570-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

FUS/TLS Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, IP, ELISA

Cat No. 11570-1-AP

Publications(126)

Reviews (9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, IP, CoIP, ChIP, RIP, ELISA

Synonyms

FUS, 75 kDa DNA pairing protein, 75 kDa DNA-pairing protein, ALS, ALS6

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: K-562 cells
• Positive IHC detected in: mouse brain tissue, human ovary tumor tissue, rat brain tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse colon tissue, mouse liver tissue
• Positive IF-Fro detected in: rat brain tissue
• Positive IF/ICC detected in: HepG2 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 10 publications below
• WB: See 65 publications below
• IHC: See 35 publications below
• IF: See 49 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below
• ChIP: See 2 publications below
• RIP: See 6 publications below

Product Information

11570-1-AP targets FUS/TLS in WB, IHC, IF/ICC, IF-P, IF-Fro, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FUS/TLS fusion protein Ag2150
• Full Name: fusion (involved in t(12;16) in malignant liposarcoma)
• Calculated Molecular Weight: 75 kDa
• Observed Molecular Weight: 68-75 kDa
• GenBank Accession Number: BC026062
• Gene Symbol: FUS/TLS
• Gene ID (NCBI): 2521
• RRID: AB_2247082
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35637
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FUS (also named TLS and POMp75) belongs to the RRM TET family. FUS may play a role in the maintenance of genomic integrity; it binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. FUS is also an RNA-binding protein, and its links to neurodegenerative disease proffer the intriguing possibility that altered RNA metabolism or RNA processing may underlie or contribute to neuron degeneration[PMID: 22640227]. FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) and is implicated in certain forms of amyotrophic lateral sclerosis (ALS) and frontotemporal dementias (FTDs) such as frontotemporal lobar dementia with ubiquitin inclusions (FTLD-U)[PMID: 22640227]. This antibody is a rabbit polyclonal antibody raised against an internal region of human FUS. FUS was detected double bands of 68-74 kDa (PMID:31519807).

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	IHC,IF	Nature; Mutations in UBQLN2 cause dominant X-linked juvenile and adult-onset ALS and ALS/dementia.; Authors - Deng Han-Xiang HX
mouse,human	WB	Nat Med; Antisense oligonucleotide silencing of FUS expression as a therapeutic approach in amyotrophic lateral sclerosis.; Authors - Vladislav A Korobeynikov
human	WB,IF	Cell; Nuclear-Import Receptors Reverse Aberrant Phase Transitions of RNA-Binding Proteins with Prion-like Domains.; Authors - Lin Guo
human	WB	Cell Metab; NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.; Authors - Mi Kyung Park
mouse	WB	Nat Neurosci; FUS-mediated regulation of acetylcholine receptor transcription at neuromuscular junctions is compromised in amyotrophic lateral sclerosis.; Authors - Gina Picchiarelli ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xiaochen (Verified Customer) (07-08-2024)

Sensitivie for IF and show image with good quelity.

• Applications: Immunofluorescence
• Cell Tissue Type: U2OS

FUS/TLS Antibody Immunofluorescence validation ( dilution) in U2OS (Cat no:11570-1-AP)

FH

Xhuljana (Verified Customer) (03-01-2024)

Used in siRNA transfected C2C12 cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: C2C12 cells

FUS/TLS Antibody Western Blot validation (1:1000 dilution) in C2C12 cells (Cat no:11570-1-AP)

FH

Zhongwen (Verified Customer) (09-25-2023)

I can find two bands in the target region. I am not sure which one is the target band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

FUS/TLS Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:11570-1-AP)

FH

manohar (Verified Customer) (07-10-2023)

Nitrocellulose membrane is used with 5% milk as blocking and antibody diluted in 1% milk and incubated overnight.

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293, Neuronal stem cells, Mouse tissue

FH

Tatyana (Verified Customer) (05-14-2023)

ICC using 5% goat serum/PBST buffer, 2 hours at RT. Good specific nuclear signal.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa

FUS/TLS Antibody Immunofluorescence validation (1:1000 dilution) in HeLa (Cat no:11570-1-AP)

FH

shashirekha (Verified Customer) (12-23-2020)

Used for immunopreciptation at 1:1000 dilution. Works very well

• Applications: Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Rat cortical neurons

FUS/TLS Antibody Immunoprecipitation validation (1:1000 dilution) in Rat cortical neurons (Cat no:11570-1-AP)

FH

H (Verified Customer) (04-06-2020)

The antibody worked well for HCT116 cell line. Nuclear cytosolic fractionation clearly showed that FUS is dominantly in nucleus, and sub-fraction is present in cytosol.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HCT116

FUS/TLS Antibody Western Blot validation (1:2000 dilution) in HCT116 (Cat no:11570-1-AP)

FH

Karthik (Verified Customer) (04-24-2019)

Magenta- FUSBlue- MAP2FUS staining consistent obtained with this antibody is consistent with literature

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:250 overnight at 4 degrees
• Cell Tissue Type: motor neurons

FH

Yen-Chen (Verified Customer) (12-03-2018)

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: human motor neuron