FUS/TLS Polyclonal antibody

• Featured Product
• KD/KO Validated

FUS/TLS Polyclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

101

Reviews (4)

Cat no : 11570-1-AP

Synonyms

75 kDa DNA pairing protein, FUS, FUS1, FUS-CHOP, hnRNP P2, Oncogene FUS, Oncogene TLS, POMp75, RNA binding protein FUS, TLS, TLS/CHOP

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Validation Data Gallery

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  WB analysis of HEK-293 using 11570-1-AP

  WB result of FUS antibody (11570-1-AP, 1:5000) with si-Control and si-FUS transfected HEK 293 cells.

• 

  WB analysis using 11570-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11570-1-AP (FUS/TLS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11570-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11570-1-AP (FUS/TLS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11570-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11570-1-AP (FUS/TLS Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 11570-1-AP

  IP Result of anti-FUS (IP:11570-1-AP, 3ug; Detection:11570-1-AP 1:1000) with K-562 cells lysate 4000ug.

• 

  IHC staining of mouse brain using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11570-1-AP (FUS/TLS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11570-1-AP (FUS/TLS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11570-1-AP (FUS/TLS antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11570-1-AP (FUS/TLS antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11570-1-AP (FUS/TLS Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11570-1-AP (FUS/TLS Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human ovary tumor using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11570-1-AP (FUS/TLS Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human ovary tumor using 11570-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11570-1-AP (FUS/TLS Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 11570-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 11570-1-AP (FUS/TLS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 11570-1-AP

  1X10^6 HeLa cells were stained with 0.2ug FUS/TLS antibody (11570-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, mouse brain tissue, HepG2 cells, SH-SY5Y cells
• Positive IP detected in: K-562 cells
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 10 publications below
• WB: See 51 publications below
• IHC: See 30 publications below
• IF: See 39 publications below
• CoIP: See 2 publications below
• ChIP: See 2 publications below
• RIP: See 4 publications below

Product Information

11570-1-AP targets FUS/TLS in WB, RIP, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FUS/TLS fusion protein Ag2150
• Full Name: fusion (involved in t(12;16) in malignant liposarcoma)
• Calculated molecular weight: 75 kDa
• Observed molecular weight: 68-75 kDa
• GenBank accession number: BC026062
• Gene symbol: FUS
• Gene ID (NCBI): 2521
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

FUS (also named TLS and POMp75) belongs to the RRM TET family. FUS may play a role in the maintenance of genomic integrity; it binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. FUS is also an RNA-binding protein, and its links to neurodegenerative disease proffer the intriguing possibility that altered RNA metabolism or RNA processing may underlie or contribute to neuron degeneration[PMID: 22640227]. FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) and is implicated in certain forms of amyotrophic lateral sclerosis (ALS) and frontotemporal dementias (FTDs) such as frontotemporal lobar dementia with ubiquitin inclusions (FTLD-U)[PMID: 22640227]. This antibody is a rabbit polyclonal antibody raised against an internal region of human FUS.

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	IHC,IF	Nature; Mutations in UBQLN2 cause dominant X-linked juvenile and adult-onset ALS and ALS/dementia.; Authors - Deng Han-Xiang HX
human,mouse	WB	Nat Med; Antisense oligonucleotide silencing of FUS expression as a therapeutic approach in amyotrophic lateral sclerosis.; Authors - Vladislav A Korobeynikov
human	WB,IF	Cell; Nuclear-Import Receptors Reverse Aberrant Phase Transitions of RNA-Binding Proteins with Prion-like Domains.; Authors - Lin Guo
human	WB	Cell Metab; NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.; Authors - Mi Kyung Park
mouse	WB	Nat Neurosci; FUS-mediated regulation of acetylcholine receptor transcription at neuromuscular junctions is compromised in amyotrophic lateral sclerosis.; Authors - Gina Picchiarelli ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

shashirekha (Verified Customer) (12-23-2020)

Used for immunopreciptation at 1:1000 dilution. Works very well

• Applications: Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Rat cortical neurons

FUS/TLS Antibody Immunoprecipitation validation (1:1000 dilution) in Rat cortical neurons (Cat no:11570-1-AP)

FH

H (Verified Customer) (04-06-2020)

The antibody worked well for HCT116 cell line. Nuclear cytosolic fractionation clearly showed that FUS is dominantly in nucleus, and sub-fraction is present in cytosol.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HCT116

FUS/TLS Antibody Western Blot validation (1:2000 dilution) in HCT116 (Cat no:11570-1-AP)

FH

Karthik (Verified Customer) (04-24-2019)

Magenta- FUSBlue- MAP2FUS staining consistent obtained with this antibody is consistent with literature

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:250 overnight at 4 degrees
• Cell Tissue Type: motor neurons

FH

Yen-Chen (Verified Customer) (12-03-2018)

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: human motor neuron

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