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  • KD/KO Validated

FUS/TLS Polyclonal antibody

FUS/TLS Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IHC, IF, FC, chIP,ELISA

Conjugate

Unconjugated

Cat no : 11570-1-AP

Synonyms

75 kDa DNA pairing protein, FUS, FUS1, FUS-CHOP, hnRNP P2, Oncogene FUS, Oncogene TLS, POMp75, RNA binding protein FUS, TLS, TLS/CHOP



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, HL-60 cells, K-562 cells, SMMC-7721 cells, human kidney tissue, HepG2 cells, mouse brain tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman breast cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11570-1-AP targets FUS/TLS in WB, RIP, IP, IHC, IF, FC, chIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FUS/TLS fusion protein Ag2150
Full Name fusion (involved in t(12;16) in malignant liposarcoma)
Calculated molecular weight 75 kDa
Observed molecular weight 68-75 kDa
GenBank accession numberBC026062
Gene symbol FUS
Gene ID (NCBI) 2521
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FUS (also named TLS and POMp75) belongs to the RRM TET family. FUS may play a role in the maintenance of genomic integrity; it binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. FUS is also an RNA-binding protein, and its links to neurodegenerative disease proffer the intriguing possibility that altered RNA metabolism or RNA processing may underlie or contribute to neuron degeneration[PMID: 22640227]. FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) and is implicated in certain forms of amyotrophic lateral sclerosis (ALS) and frontotemporal dementias (FTDs) such as frontotemporal lobar dementia with ubiquitin inclusions (FTLD-U)[PMID: 22640227]. This antibody is a rabbit polyclonal antibody raised against an internal region of human FUS.

Protocols

Product Specific Protocols
WB protocol for FUS/TLS antibody 11570-1-APDownload protocol
IHC protocol for FUS/TLS antibody 11570-1-APDownload protocol
IF protocol for FUS/TLS antibody 11570-1-APDownload protocol
IP protocol for FUS/TLS antibody 11570-1-APDownload protocol
FC protocol for FUS/TLS antibody 11570-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Exp Clin Cancer Res

FUS/circ_002136/miR-138-5p/SOX13 feedback loop regulates angiogenesis in Glioma.

Authors - Zhenwei He
mouseIF

Hum Mol Genet

Quantitative proteomics identifies proteins that resist translational repression and become dysregulated in ALS-FUS.

Authors - Desiree M Baron
humanchIP

FEBS Lett

LINC00473 mediates cyclin D1 expression through a balance between activation and repression signals in breast cancer cells.

Authors - Xiangmin Shi
mouseIF

Acta Neuropathol Commun

Restoration of histone acetylation ameliorates disease and metabolic abnormalities in a FUS mouse model.

Authors - Elisabeth Rossaert
humanIHC

Acta Neuropathol Commun

Phosphorylated TDP-43 (pTDP-43) aggregates in the axial skeletal muscle of patients with sporadic and familial amyotrophic lateral sclerosis.

Authors - Matthew D Cykowski
humanWB,IF

Cell Rep

Antiviral Immune Response as a Trigger of FUS Proteinopathy in Amyotrophic Lateral Sclerosis.

Authors - Tatyana A Shelkovnikova
  • KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

shashirekha (Verified Customer) (12-23-2020)

Used for immunopreciptation at 1:1000 dilution. Works very well

  • Applications: Immunoprecipitation
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Rat cortical neurons
FUS/TLS Antibody Immunoprecipitation validation (1:1000 dilution) in Rat cortical neurons (Cat no:11570-1-AP)
FH

H (Verified Customer) (04-06-2020)

The antibody worked well for HCT116 cell line. Nuclear cytosolic fractionation clearly showed that FUS is dominantly in nucleus, and sub-fraction is present in cytosol.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HCT116
FUS/TLS Antibody Western Blot validation (1:2000 dilution) in HCT116 (Cat no:11570-1-AP)
FH

Karthik (Verified Customer) (04-24-2019)

Magenta- FUSBlue- MAP2FUS staining consistent obtained with this antibody is consistent with literature

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:250 overnight at 4 degrees
  • Cell Tissue Type: motor neurons
FH

Yen-Chen (Verified Customer) (12-03-2018)

  • Applications: Western Blot,Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: human motor neuron