FXR2 Polyclonal antibody

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  WB analysis of HeLa using 12552-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12552-1-AP (FXR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 12552-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12552-1-AP (FXR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12552-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12552-1-AP (FXR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12552-1-AP

  IP result of anti-FXR2 (IP:12552-1-AP, 4ug; Detection:12552-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of mouse brain using 12552-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12552-1-AP (FXR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12552-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12552-1-AP (FXR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 12552-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FXR2 antibody (12552-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of HepG2 using 12552-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FXR2 antibody (12552-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• Featured Product
• KD/KO Validated

FXR2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12552-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Synonyms

RNA-binding protein FXR2, FXR2P, FMR1L2, FMR1 autosomal homolog 2

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, human brain tissue, mouse brain tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 2 publications below
• RIP: See 1 publications below

Product Information

12552-1-AP targets FXR2 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3402

  Product name: Recombinant human FXR2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-354 aa of BC020090

  Sequence: MGGLASGGDVEPGLPVEVRGSNGAFYKGFVKDVHEDSVTIFFENNWQSERQIPFGDVRLPPPADYNKEITEGDEVEVYSRANEQEPCGWWLARVRMMKGDFYVIEYAACDATYNEIVTLERLRPVNPNPLATKGSFFKVTMAVPEDLREACSNENVHKEFKKALGANCIFLNITNSELFILSTTEAPVKRASLLGDMHFRSLRTKLLLMSRNEEATKHLETSKQLAAAFQEEFTVREDLMGLAIGTHGANIQQARKVPGVTAIELGEETCTFRIYGETPEACRQARSYLEFSEDSVQVPRNLVGKVIGKNGKVIQEIVDKSGVVRVRVEGDNDKKNPREEGMVPFIFVGTRENI

• Full Name: fragile X mental retardation, autosomal homolog 2
• Calculated Molecular Weight: 74 kDa
• Observed Molecular Weight: 95 kDa
• GenBank Accession Number: BC020090
• Gene Symbol: FXR2
• Gene ID (NCBI): 9513
• RRID: AB_2110720
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P51116
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FXR2 (FMR1 autosomal homolog 2), also known as: FXR2P; Fxr2h, is belong to the Fragile X gene family. FXR2 is a mRNA-binding protein that acts as a regulator of mRNAs translation and/or stability, and which is required for adult hippocampal neurogenesis. Also, FXR2 acts as a regulator of adult hippocampal neurogenesis by regulating translation and/or stability of NOG mRNA, thereby preventing NOG protein expression in the dentate gyrus(Uniprot).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB,IF	Adv Sci (Weinh); Primate-Specific DAZ Regulates Translation of Cell Proliferation-Related mRNAs and is Essential for Maintenance of Spermatogonia; Authors - Ningjing Ou
human	WB,IF	Int J Biol Macromol; 5-Methylcytosine-modified circRNA-CCNL2 regulates vascular remdeling in hypoxic pulmonary hypertension through binding to FXR2; Authors - Hanliang Sun
human	WB	J Cell Biol; UBAP2L ensures homeostasis of nuclear pore complexes at the intact nuclear envelope; Authors - Yongrong Liao
human	RIP	Elife; The Tudor SND1 protein is an m6A RNA reader essential for replication of Kaposi's sarcoma-associated herpesvirus.; Authors - Belinda Baquero-Perez
human	WB	Proc Natl Acad Sci U S A; ARL6IP1 mediates small-molecule-induced alleviation of Alzheimer pathology through FXR1-dependent BACE1 translation initiation; Authors - Gui-Feng Zhou ; KD Validated
Mouse	WB	J Nanobiotechnology; Anti-ferroptosis exosomes engineered for targeting M2 microglia to improve neurological function in ischemic stroke; Authors - Yong Wang