FXYD6 Polyclonal antibody

• Featured Product
• KD/KO Validated

FXYD6 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15805-1-AP

Synonyms

FXYD6, Phosphohippolin

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Validation Data Gallery

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  WB analysis using 15805-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15805-1-AP (FXYD6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 15805-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15805-1-AP (FXYD6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15805-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15805-1-AP (FXYD6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 15805-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15805-1-AP (FXYD6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 15805-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 15805-1-AP (FXYD6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15805-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15805-1-AP (FXYD6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 15805-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15805-1-AP (FXYD6 antibody) at dilution of 1:100 (under 40x lens).

• 

  FC experiment of SH-SY5Y using 15805-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug FXYD6 antibody (15805-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: PC-12 cells, mouse brain tissue, human brain tissue, human testis tissue, rat brain tissue, SH-SY5Y cells
• Positive IHC detected in: human brain tissue, human liver tissue, human ovary tissue, human skin tissue, human spleen tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

15805-1-AP targets FXYD6 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FXYD6 fusion protein Ag8538
• Full Name: FXYD domain containing ion transport regulator 6
• Calculated molecular weight: 95 aa, 11 kDa
• Observed molecular weight: 20 kDa
• GenBank accession number: BC018652
• Gene symbol: FXYD6
• Gene ID (NCBI): 53826
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The FXYD family is a group of small single-span transmembrane proteins characterized by a signature sequence containing an FXYD motif, two conserved glycines and a serine residue. Members of the FXYD family, including FXYD1 (phospholemman), FXYD2 (gamma subunit of Na,K-ATPase), FXYD3 (Mat8), FXYD4 (CHIF), FXYD5 (RIC), FXYD6 (phosphohippolin) and FXYD7, are tissue specific regulators of the Na,K-ATPase. FXYD6 is primarily expressed in the brain. It modulates the kinetic activity of Na,K-ATPase and has long-term physiological importance in maintaining cation homeostasis. It may play a role in endolymph composition and has a potential important role in neuronal excitability of the CNS during postnatal development and in the adult brain. On the SDS-PAGE FXYD6 migrates with an apparent molecular weight of approximately 20 kDa, which is larger than the calculated molecular weight of 10.5 kDa (PMID: 15193427; 17209044). The gene encodes FXYD6 is located on chromosome 11q23.3, and it might be a susceptibility gene of schizophrenia.

Publications

Species	Application	Title
mouse	IF	J Am Soc Nephrol; Single-Cell RNA Sequencing Reveals Renal Endothelium Heterogeneity and Metabolic Adaptation to Water Deprivation.; Authors - Sébastien J Dumas
human	WB	Biomed Res Int; FXYD6 Regulates Chemosensitivity by Mediating the Expression of Na+/K+-ATPase α1 and Affecting Cell Autophagy and Apoptosis in Colorectal Cancer.; Authors - Wen Luo ; KD Validated

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