Fibrinogen gamma chain Recombinant monoclonal antibody

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  WB analysis using 84616-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84616-4-RR (Fibrinogen gamma chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84616-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84616-4-RR against Mouse Fibrinogen gamma chain were performed. The affinity constant is 0.677 nM.

Fibrinogen gamma chain Recombinant Antibody for WB, ELISA

Cat No. 84616-4-RR

Clone No.241958B3

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

Fgg, 241958B3

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Tested Applications

• Positive WB detected in: mouse spleen tissue, mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84616-4-RR targets Fibrinogen gamma chain in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: fibrinogen gamma chain
• Calculated Molecular Weight: 49kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: NM_133862.2
• Gene Symbol: Fgg
• Gene ID (NCBI): 99571
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q8VCM7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibrinogen gamma chain, also known as Fgg. It is an isomeric hexamer, which is linked by disulfide bonds. It contains two groups of three different chains (α, β and γ). The two heterotrimers are head-to-head conformation, and the N-terminal is in a small central domain (by similarity). It is polymerized with fibrinogen α(FGA) and fibrinogen β(FGB) to form insoluble fibrin matrix. In addition, it plays a role in stabilizing injury in the early stage of wound repair and guiding cell migration in the process of epithelial re-formation. The molecular weight of Fgg is 50 kDa.