Flightless I Polyclonal antibody

Flightless I Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27729-1-AP

Synonyms

FLI, Fli1, Flightless-1, FLII, FLIL, Protein flightless 1 homolog

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Validation Data Gallery

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  WB analysis using 27729-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27729-1-AP (Flightless I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 27729-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27729-1-AP (Flightless I antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 27729-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27729-1-AP (Flightless I antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 27729-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Flightless I antibody (27729-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: MCF-7 cells, T-47D cells, THP-1 cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27729-1-AP targets Flightless I in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Flightless I fusion protein Ag26865
• Full Name: flightless I homolog (Drosophila)
• Calculated Molecular Weight: 1269 aa, 145 kDa
• Observed Molecular Weight: 145-150 kDa
• GenBank Accession Number: BC025300
• Gene Symbol: FLII
• Gene ID (NCBI): 2314
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Flightless I (FliI) is the most evolutionarily conserved member of the gelsolin superfamily of proteins which are key regulators of actin filament assembly and turnover. FliI comprises an N-terminal leucine-rich repeat (LRR) domain which is not present in other gelsolin family members, and the LRR domain may enable interactions between FliI and other molecules involved in signal transduction, thereby spatially integrating signaling and actin remodeling functions. This protein was originally found in Drosophila and participates in the embryonic development, while mammalian FliI protein was involved in the regulation of wound repair,skin barrier development. Studies recently demonstrated that FliI protein associated with colorectal cancer, hepatocellular and prostate cancer (PMID:30091651; 28498392).