Flow Cytometry Staining Buffer (1X)

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  1x10^6 human PBMCs were washed and resuspended in Flow Cytometry Staining Buffer (PF00018) and surface stained with 5 uL APC CD3 Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed. Single cells were gated.

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  1x10^6 human PBMCs were washed and resuspended in Flow Cytometry Staining Buffer (PF00018) and surface stained with 5 uL PE Anti-Human CD14 (26IC) Rabbit IgG RecAb (PE-FcA65616, Clone: 26IC). Cells were not fixed. Single cells were gated.

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  1x10^6 human PBMCs were washed and resuspended in Flow Cytometry Staining Buffer (PF00018) and surface stained with 5 uL APC-Cyanine7 Anti-Human CD45 Rabbit Recombinant Antibody (AY7-98117, Clone: 241670E10). Cells were not fixed. Single cells were gated.

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Cat No. PF00018

Publications

12

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Product Information

Proteintech's Flow Cytometry Staining Buffer (1X, #PF00018) is a buffer formulated for use in flow cytometry. It is suitable for all wash and incubation steps required for dilution of antibodies, dilution of cells, and surface staining for flow cytometry analysis. It maximizes the maintenance of cell activity and minimizes non-specific staining. This product is a 1× working solution and is ready to use.

Component	Volume
Flow Cytometry Staining Buffer (1X)	500 mL

Note: This product contains fetal bovine serum and sodium azide (≤0.09%).

Storage

Keep sealed and protected from light at 4°C. Valid for 1 year.

Usage

1. After collecting the cell samples into a flow tube, add Flow Cytometry Staining Buffer to 2 mL and centrifuge at 300-400 g for 5 minutes, then discard the supernatant.

2. Repeat step 1.

3. Add the appropriate amount of Flow Cytometry Staining Buffer as required for the experiment.

4. Dispense the cell samples into each experimental group of flow tubes in the volume and cell concentration required for the experiment.

5. Stain the cell surface antigens at the optimal concentration recommended in the antibody instructions.

6. After staining is complete, add Flow Cytometry Staining Buffer to 2 mL to wash the cells, centrifuge at 300-400 g for 5 minutes, and discard the supernatant.

7. Repeat step 6.

8. Add 0.2 mL Flow Cytometry Staining Buffer and resuspend the cell precipitate in Flow Cytometry Staining Buffer.

9. Collect samples on a Flow Cytometer.

Cautions

This product contains sodium azide and requires personal protective measures when used, such as wearing gloves and lab coats to avoid contact with the eyes or skin.

Cited in Article as

PF00018, Flow Cytometry Staining Buffer (1X), Proteintech, IL, USA

Publications

Application	Title
FC	Nat Aging; Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer; Authors - Yifei Cheng
	J Immunother Cancer; Hypoxia upregulates the expression of PD-L1 via NPM1 in breast cancer; Authors - Yihui Yu
FC	Biochem Pharmacol; Targeting HMGA1 contributes to immunotherapy in aggressive breast cancer while suppressing EMT; Authors - Xing Chang
FC	Int J Mol Sci; Establishment of a Novel Platform for Developing Oral Vaccines Based on the Surface Display System of Yeast Spores; Authors - Chenyu Si
	Cells; The Six-Transmembrane Epithelial Antigen of the Prostate (STEAP) 3 Regulates the Myogenic Differentiation of Yunan Black Pig Muscle Satellite Cells (MuSCs) In Vitro via Iron Homeostasis and the PI3K/AKT Pathway; Authors - Wei Zhang
	In Vitro Cell Dev Biol Anim; PKCβ expression contributes to M1 macrophage-induced impairment in the osteogenic differentiation of periodontal ligament stem cells; Authors - Yang Liu