G-CSF Recombinant monoclonal antibody, PBS Only (Capture)

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  WB analysis using 86376-1-RR (same clone as 86376-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86376-1-RR (G-CSF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86376-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP02556-1

  Serum of eight individual healthy human donors was measured. The G-CSF concentration of detected samples was determined to be 40.3 pg/mL with a range of 11.8-197.6 pg/mL.

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  Sandwich ELISA sample test of MP02556-1

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days. The mean G-CSF concentration was determined to be 33.9 pg/mL in unstimulated PBMC supernatant, 858.2 pg/mL in PHA stimulated PBMC supernatant.

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  Sandwich ELISA standard curve of MP02556-1

  Sandwich ELISA standard curve of MP02556-1, Human G-CSF Recombinant Matched Antibody Pair - PBS only. 86376-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard HZ-1207. 86376-2-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL.

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  IF Staining of K-562 using 86376-1-RR (same clone as 86376-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using G-CSF antibody (86376-1-RR, Clone: 250389H1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86376-1-PBS in a different storage buffer formulation.

• 

  FC experiment of K-562 using 86376-1-RR (same clone as 86376-1-PBS)

  1x10^6 K-562 cells were intracellularly stained with 0.25 ug G-CSF Recombinant antibody (86376-1-RR, Clone:250389H1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 86376-1-PBS in a different storage buffer formulation.

G-CSF Uni-rAb^® Recombinant Antibody for WB, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA

Cat No. 86376-1-PBS

Clone No.250389H1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA

Synonyms

CSF3, C17orf33, CSF 3, FilgrastimLenograstim, GCSF

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Product Information

86376-1-PBS targets G-CSF as part of a matched antibody pair:

MP02556-1: 86376-1-PBS capture and 86376-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo:

  Product name: HumanKine® recombinant human G-CSF protein

  Source: -derived,

  Tag:

  Domain: of

  Sequence:

• Full Name: colony stimulating factor 3 (granulocyte)
• Observed Molecular Weight: 20 kDa
• Gene Symbol: G-CSF
• Gene ID (NCBI): 1440
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P09919
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Granulocyte colony-stimulating factor (G-CSF), also referred to as CSF3, is a protective cytokine with anti-inflammatory effects. G-CSF is important in promoting survival of the granulocytic lineage cells and proliferation and migration of neutrophils as well as trophoblast cells. G-CSF acts by binding to its receptor G-CSFR (also called CSF3R), which after binding with G-CSF activates the canonical Janus kinase (Jak)/signal transducer, activator of transcription (STAT)and Ras/Raf/MAP kinase pathways. G-CSF potently stimulates the proliferation and release of peripheral blood progenitor cells into the bloodstream and is therefore used to treat neutropenia after chemotherapy. Furthermore, G-CSF levels are elevated upon intensive exercise leading to increased neutrophil counts, which are predominantly due to delayed neutrophil apoptosis.