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  • KD/KO Validated

GAD1 Polyclonal antibody

GAD1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 10408-1-AP


GAD, GAD 67, GAD1, GAD67, Glutamate decarboxylase 1, SCP

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10408-1-AP targets GAD1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GAD1 fusion protein Ag0666
Full Name glutamate decarboxylase 1 (brain, 67kDa)
Calculated Molecular Weight 67 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC002815
Gene Symbol GAD1
Gene ID (NCBI) 2571
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The GAD1(glutamate decarboxylase 1) gene encodes 67 kDa glutamic acid decarboxylase isoform (GAD67), the rate-limiting enzyme responsible for γ-aminobutyric acid (GABA) biosynthesis from glutamatic acid and the major GAD isoform in the human brain for early brain development (PMID:21302352). It is also potentially involved in variety of skin activities. It belongs to the group II decarboxylase family.


Product Specific Protocols
WB protocol for GAD1 antibody 10408-1-APDownload protocol
IHC protocol for GAD1 antibody 10408-1-APDownload protocol
IF protocol for GAD1 antibody 10408-1-APDownload protocol
IP protocol for GAD1 antibody 10408-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Cell Biol

Cancer-cell-derived GABA promotes β-catenin-mediated tumour growth and immunosuppression.

Authors - De Huang
  • KD Validated

Sci Adv

RasGRP1 is a causal factor in the development of l-DOPA-induced dyskinesia in Parkinson's disease.

Authors - Mehdi Eshraghi

Sci Rep

Renal Denervation Improves the Baroreflex and GABA System in Chronic Kidney Disease-induced Hypertension.

Authors - Hsin-Hung Chen

Transl Psychiatry

Long non-coding RNA Neat1 regulates adaptive behavioural response to stress in mice.

Authors - Michail S Kukharsky

Environ Toxicol

Perinatal exposure to bisphenol A impairs cognitive function via the gamma-aminobutyric acid signaling pathway in male rat offspring

Authors - Yi Guo

Front Pharmacol

KangPiLao decoction modulates cognitive and emotional disorders in rats with central fatigue through the GABA/Glu pathway

Authors - Yifei Xu


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Lana (Verified Customer) (02-06-2020)

Besides 67 kDa non-specific bands are present.SDS-PAGE: 15 ug/ul RIPA lysate of whole striatal tissue. 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking: SEA Block Blocking Buffer 1hPrimary Ab: O/N incubation at 4C, 1:1000Secondary Ab: IRDye 800CW Goat anti-Rabbit, 1:15000.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Brain tissue, human.
GAD1 Antibody Western Blot, validation (1:1000 dilution) in Brain tissue, human. (Cat no:10408-1-AP)