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  • KD/KO Validated

GAD1 Polyclonal antibody

GAD1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10408-1-AP

Synonyms

GAD, GAD 67, GAD1, GAD67, Glutamate decarboxylase 1, SCP



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10408-1-AP targets GAD1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GAD1 fusion protein Ag0666
Full Name glutamate decarboxylase 1 (brain, 67kDa)
Calculated molecular weight 67 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC002815
Gene symbol GAD1
Gene ID (NCBI) 2571
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The GAD1(glutamate decarboxylase 1) gene encodes 67 kDa glutamic acid decarboxylase isoform (GAD67), the rate-limiting enzyme responsible for γ-aminobutyric acid (GABA) biosynthesis from glutamatic acid and the major GAD isoform in the human brain for early brain development (PMID:21302352). It is also potentially involved in variety of skin activities. It belongs to the group II decarboxylase family.

Protocols

Product Specific Protocols
WB protocol for GAD1 antibody 10408-1-APDownload protocol
IHC protocol for GAD1 antibody 10408-1-APDownload protocol
IP protocol for GAD1 antibody 10408-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Nat Cell Biol

Cancer-cell-derived GABA promotes β-catenin-mediated tumour growth and immunosuppression.

Authors - De Huang
  • KD Validated
ratWB

Sci Rep

Renal Denervation Improves the Baroreflex and GABA System in Chronic Kidney Disease-induced Hypertension.

Authors - Hsin-Hung Chen
mouse

Transl Psychiatry

Long non-coding RNA Neat1 regulates adaptive behavioural response to stress in mice.

Authors - Michail S Kukharsky
mouseWB

Front Pharmacol

BNSTAVGABA-PVNCRF Circuit Regulates Visceral Hypersensitivity Induced by Maternal Separation in Vgat-Cre Mice.

Authors - Si-Ting Huang
mouseWB

Front Pharmacol

Maternal Nicotine Exposure Alters Hippocampal Microglia Polarization and Promotes Anti-inflammatory Signaling in Juvenile Offspring in Mice.

Authors - Li Zhou
ratWB

Front Pharmacol

KangPiLao decoction modulates cognitive and emotional disorders in rats with central fatigue through the GABA/Glu pathway

Authors - Yifei Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Lana (Verified Customer) (02-06-2020)

Besides 67 kDa non-specific bands are present.SDS-PAGE: 15 ug/ul RIPA lysate of whole striatal tissue. 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking: SEA Block Blocking Buffer 1hPrimary Ab: O/N incubation at 4C, 1:1000Secondary Ab: IRDye 800CW Goat anti-Rabbit, 1:15000.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Brain tissue, human.
GAD1 Antibody Western Blot, validation (1:1000 dilution) in Brain tissue, human. (Cat no:10408-1-AP)