GAD1 Polyclonal antibody

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  WB analysis using 10408-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10408-1-AP (GAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10408-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10408-1-AP (GAD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10408-1-AP

  IP result of anti-GAD1 (IP:10408-1-AP, 4ug; Detection:10408-1-AP 1:500) with mouse brain tissue lysate 1920 ug.

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  IHC staining of mouse brain using 10408-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10408-1-AP (GAD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10408-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10408-1-AP (GAD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10408-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10408-1-AP (GAD1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10408-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10408-1-AP (GAD1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 10408-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10408-1-AP (GAD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 10408-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10408-1-AP (GAD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 10408-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using GAD1 antibody (10408-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

GAD1 Polyclonal Antibody for WB, IHC, IF-Fro, IP, ELISA

Cat No. 10408-1-AP

Publications(32)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-Fro, IP, ELISA

Synonyms

GAD67, GAD 67, GAD 1, GAD, FLJ45882

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue, mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-Fro detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 24 publications below
• IHC: See 5 publications below
• IF: See 4 publications below

Product Information

10408-1-AP targets GAD1 in WB, IHC, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0666

  Product name: Recombinant human GAD1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-224 aa of BC002815

  Sequence: MASSTPSSSATSSNAGADPNTTNLRPTTYDTWCGVAHGCTRKLGLKICGFLQRTNSLEEKSRLVSAFKERQSSKNLLSCENSDRDARFRRTETDFSNLFARDLLPAKNGEEQTVQFLLEVVDILLNYVRKTFDRSTKVLDFHHPHQLLEGMEGFNLELSDHPESLEQILVDCRDTLKYGVRTGHPRFFNQLSTGLDIIGLAGEWLTSTANTNMPSDMRECWLLR

• Full Name: glutamate decarboxylase 1 (brain, 67kDa)
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC002815
• Gene Symbol: GAD1
• Gene ID (NCBI): 2571
• RRID: AB_2107733
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99259
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The GAD1(glutamate decarboxylase 1) gene encodes 67 kDa glutamic acid decarboxylase isoform (GAD67), the rate-limiting enzyme responsible for γ-aminobutyric acid (GABA) biosynthesis from glutamatic acid and the major GAD isoform in the human brain for early brain development (PMID:21302352). It is also potentially involved in variety of skin activities. It belongs to the group II decarboxylase family.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human,mouse	WB	Nat Cell Biol; Cancer-cell-derived GABA promotes β-catenin-mediated tumour growth and immunosuppression.; Authors - De Huang ; KD Validated
mouse	WB	Sci Adv; RasGRP1 is a causal factor in the development of l-DOPA-induced dyskinesia in Parkinson's disease.; Authors - Mehdi Eshraghi
mouse	WB	PLoS Biol; Mdga2 deficiency leads to an aberrant activation of BDNF/TrkB signaling that underlies autism-relevant synaptic and behavioral changes in mice; Authors - Dongdong Zhao
rat	WB	Sci Rep; Renal Denervation Improves the Baroreflex and GABA System in Chronic Kidney Disease-induced Hypertension.; Authors - Hsin-Hung Chen
mouse		Transl Psychiatry; Long non-coding RNA Neat1 regulates adaptive behavioural response to stress in mice.; Authors - Michail S Kukharsky
mouse	WB,IF	Front Mol Neurosci; Follicle-stimulating hormone induces depression-like phenotype by affecting synaptic function; Authors - Liqin Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Lana (Verified Customer) (02-06-2020)

Besides 67 kDa non-specific bands are present.SDS-PAGE: 15 ug/ul RIPA lysate of whole striatal tissue. 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking: SEA Block Blocking Buffer 1hPrimary Ab: O/N incubation at 4C, 1:1000Secondary Ab: IRDye 800CW Goat anti-Rabbit, 1:15000.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Brain tissue, human.

GAD1 Antibody Western Blot, validation (1:1000 dilution) in Brain tissue, human. (Cat no:10408-1-AP)